| Objective:To observe the expression of desmin of podocyte in Focal segmental glomerulosclerosis rats which was constructed with one side nephrectomy and injection of Adriamycin caudal-venously respectively,and apply valsartan for intervention.To investigate the role of podocyte injury in the occurrence and development of focal segmental glomerulosclerosis and the protective effect of valsartan on podocyte.Methods:21 male Wistar rats were randomized to three groups:normal control group(A,n=6),Valsartan-treated group(B,n=8),and model group(C,n=8).Rat model of FSGS was constructed with one side nephrectomy and injection of Adriamycin caudal-venously respectively.24-hours'urinary protein and blood biochemical indicators were measured at the 0,4th,6th,8th week,and creatinine clearance rate(Ccr) was calculated.Renal pathological changes were evaluated at 8th week.Expression of desmin was detected by immunohistochemical method.Results:1.The changes of various biochemical index:①24-hours'urinary protein:24hUP in FSGS group was 2 times higher than the original data at the 4th week(P＜0.001),and it was 3 times at the 8th week(P＜0.001).24hUP in FSGS group and Valsartan-treated group were higher than that of normal control group at each experimental period(P＜0.05).24hUP in Valsartan-treated group was lower than that of FSGS group at the 6th week(P＜0.05),and it decreased significantly at the 8th week(P＜0.01).②Serum albumin and cholesterol:albumin and cholesterol in FSGS rats had significant difference among all periods(P＜0.001).Albumin at the 6th week was lower than the 4th week(P＜0.05)as well as the 8th week and the 6th week(P＜0.01).Cholesterol at the 6th week was higher than the 4th week(P＜0.01),but the difference between the 8th and 6th week was no statistically significant (P＞0.05).③Creatinine clearance rate(Ccr):Ccr in FSGS group and Valsartan-treated group were lower than that of normal control group at each experimental period(P＜0.001).At the 8th week,Ccr in Valsartan-treated group was higher than that of FSGS group(P＜0.01).2.Changes of renal pathology:①Light microscopic observation:the pathologic changes in FSGS group were obvious,it showed sclerosis,mesangial matrix proliferation,adhesion,glomerular hypertrophy,protein cast and infiltration of inflammatory cells.The pathologic changes in Valsartan-treated group were better than FSGS group,the glomerular capillary loop opened better and the tubulointerstitial lesions lessened.②Pathological scores of glomerulus and tubular-interstitium:the in FSGS group were all higher than those of normal control group(P＜0.001).③SI and ECM/GA in FSGS group and Valsartan-treated group were higher than those of normal control group(P＜0.001,P＜0.01).SI and ECM/GA in Valsartan-treated group was lower than those of FSGS group(P＜0.001).3.The expression of desmin in FSGS group and Valsartan-treated group were higher than that of normal control group(P＜0.001).The expression of desmin in Valsartan-treated group was lower significantly than those of FSGS group(P＜0.001).4.The expression of desmin in FSGS group was positively correlated to 24hUP,pathological scores of glomerulus and tubular-interstitium(r=0.910,0.932,0.871,P＜0.05),it was negatively correlated to Ccr(r=-0.927,P＜0.01),and there were no correlation with serum albumin and cholesterol(r=0.371,0.710,P＞0.05).Conclusions:1.The rat model of FSGS constructed with one side-nephrectomy and duplicate injection Adriamycin is successful.2.Podocyte injury plays an important role in occurrence and development of FSGS.Desmin can be used as a sensitive index of podocyte injury in FSGS.Agument of the expression of desmin maybe one of the important factors in occurrence and development of FSGS3.ARB-valsartan can regulate the expression of desmin to protect podocyte,and then relieve FSGS. |
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