Effects Of Insulin-like Growth Factorâ…  And Transforming Growth Factor-β₁ On Cell Proliferation And Differentiation Of Human Osteoblast-like Cell Cultured In Vitro

Objective:To investigate the effects of single and combined use of insulin-like growth factgorâ… (IGF-â… )and transforming growth factorβ₁(TGF-β₁)on the proliferation and differentiation of human osteoblast-like cell MG-63 cultured in vitro.Methods:1.MG-63 cells cultured in vitro were treated with different dosages of rhIGF-â… (0.1,1.0,10.0,100.0 ng/ml) or rhTGF-β₁(0.01,0.1,1.0,10.0ng/ml).Cell proliferation was measured by methylthiazol tetrazolium(MTT)colorimetric method to choose an optimal dosage of rhIGF-â… and rhTGF-β₁.2.MTT method and alkaline phosphatase(ALP)measurement kit were used to determine the proliferation at different time points(1,3,5 and 7 days)and ALP activity of MG-63 cell after optimal dosage of rhlGF-â… or/and rhTGF-β₁ were added into the culture medium.Results:1.Compared with control group,both IGF-â… and TGF-β₁ significantly promoted the proliferation of MG-63 (P＜0.05);The cell number in osteoblasts exposed to IGF-â… -treated cells was higher than that in TGF-β₁-treated cells (P＜0.05);2.When using different dosages,10ng/ml IGF-â… and 1ng/ml TGF-β₁ showed the strongest effects of promoting proliferation(P＜0.05);3.The combined group of IGF-â… (10ng/ml) and TGF-β₁(1ng/ml)showed an even higher proliferative effect than using them respectively.4.Compared with control group, significant changes of cellular ALP activities were found when using IGF-â… or TGF-β₁ while combining them showed stronger effects.Conclusion:The combined use of IGF-â… and TGF-β₁ in a proper concentration significantly enhances the proliferation and differentiation of human osteoblasts cultured in vitro.