The Influence Of Platelet-derived Growth Factor And Transforming Growth Factor On Osteoblast In Vitro

ObjectivePlatelet-derived growth factor (PDGF) and transforming growth factor (TGF-β) alone or in combination in vitro osteoblast proliferation and differentiation.MethodsRemove Health 24h Wistar rats calvaria bone, cut into pieces after shaving the net, using modified tissue digestion enzyme mixed culture of osteoblasts, Different concentrations of TGF, PDGF join the 3rd generation of osteoblasts in cell culture medium to continue,7 days after the determination by MTT cell proliferation, Alkaline phosphatase detection kit osteoblast alkaline phosphataseactivity, immunohistochemical staining to detect the amount of collagen typeⅠ.ResultsPDGF and TGF-βhave contributed to a certain degree of proliferation of bone cells from the numerical point of view both PDGF proliferation-promoting stronger, combined with the highest group, SPSS statistical analysis results:the difference was statistically significant (P＜0.05). As shown, the control group the lowest ALP activity, PDGF alone group was slightly higher, followed by TGF-βalone group, combined with the highest activity group of ALP. Alone group and control group, A significant difference between the value (P＜0.05); combined group and alone group and control group, the differences were significant (P＜0.05). ConclutionsPDGF and TGF have contributed to a certain degree of bone cell proliferation and differentiation effect, the two joint application has a good synergy.