Effects Of Geldanamycin On The Proliferation And Apoptosis In Human Gastric Carcinoma SGC-7901 Cells

Objective: To investigate the effects of Geldanamycin on the proliferation and apoptosis in human gastric carcinoma SGC-7901 cells,and approach the possible mechanisms.Methods: The human gastric carcinoma SGC-7901 cell line was incubated with different concentrations of geldanamycin for different time,MTT assay for cell growth and viability,the cell apoptosis was measured by flow cymetory using anexin V/PI; immunocytochemistory was taken to test the expression of P-Akt and P-ERK;Results:After the human gastric carcinoma cell SGC-7901 were interfered by geldanamycin, the proliferation of cells was depressed in time and concentration-dependent manner; the cell apoptosis percentage was increased obviously;the expressions of P-Akt and P-ERK were decreased.Conclusions: Geldanamycin can inhibit proliferation and induce apoptosis of human gastric carcinoma cell SGC-7901 by down-regulating the protein content of P-Akt and P-ERK.Taken together, these data suggest that geldanamycin may be useful as an adjuvant therapy in gastric carcinoma.