Experimental Study Of Biocompatibility Of Bioceramic Materials Of Hydroxyapatite

Congenital malformation and rheumatic heart disease, heart degradation and acquired heart valve disease caused by bacterial infections will lead to heart valve dysfunction, and seriously threat to human life. The last treatment of the disease is the artificial heart valve replacement. Mechanical valves have been widely used in the current artificial heart valve replacement due to the better durability. The need of lifelong anticoagulant therapy is the biggest problem to Mechanical valve in its clinical application, which is undoubtedly in the physical and mental suffering but also the economic burden to the patient.Hydroxyapatite, similar to the calcium phosphate minerals in the biological bone tissue, has good biocompatibility, and is a biomedical activity ceramic material with great prospect. As the poor mechanical properties, brittle, low-intensity of purely HA, the field of application of it will be limited greatly. Use the pulsed laser deposition to prepare hydroxyapatite film on the mechanical valve, and use cell growing techniques to cultivate the vascular endothelial cells on the hydroxyapatite film, then the surface of mechanical valve will be the vascular endothelial cells and the artificial valve with the advantages of the mechanical valve but also the bioprosthetic valve will be got. This is the idea of our team to improve the film hydroxyapatite mechanical valve blood compatibility.In this paper, my job is the study of the early basic research. The primary work is to study the biocompatibility of hydroxyapatite target and lay the foundation of the preparation of high-quality hydroxyapatite films. Three temperature hydroxyapatite targets, 300℃, 600℃, 900℃, were prepared. XRD test results showed that the targets sintered under the three temperatures are still preserve the structure and composition of the hydroxyapatite. Biocompatibility test results showed that the targets under the three temperature have no significant impact on the shape, activity of human umbilical vein endothelial cells and the proliferation. And the targets have no significant toxicity to the vascular endothelial cells, their cytocompatibility are good. The hydroxyapatite targets of three temperature and the endothelial cells formed on the surface of the stuff after human umbilical vein endothelial cells cultured in the composite material, make the composite material with good anti-clotting properties and hemocompatibility. And the test results also showed that the biocompatibility of the hydroxyapatite target sintered under the three temperatures have no significant difference.