Preparation And Cytocompatibility Of Polylacticacid/Hydroxyapatite/Graphene Oxide Nanocomposites

Tissue engineering is a field of research in biomedical engineering that applies the principles of engineering and life sciences to improve tissue biologic function. As an important research field of tissue engineering, biomaterials are widely used in the clinic. Currently, synthetic biomaterials (bioceramics and biopolymers) have been widely applied to the tissue engineering fields. Poly lactic acid (PLA), with desirable biocompatibility and biodegradability, is one of the most promising biodegradable polymers for tissue engineering. Hydroxyapatite (HA), as a kind of biomaterials, exhibits excellent biocompatibility, bioactivity and osteoconductivity. HA has been considered as one of ideal scaffolds for bone tissue engineering. Also, graphene Oxide (GO), with extraordinary mechanical properties (high Young's modulus and hardness, and excellent flexibility), has been considered to be effective reinforcements for high-performance composites. Moreover, GO have has been proved to exhibit excellent biocompatibility.It is well known that the electrospinning technique can be used to produce biocompatible polymer fibers with diameters from nanometer to micrometer. Recently, the electrospinning technique has been applied for tissue engineering. It not only fabricates scaffolds that mimic the natural extra cellular matrix (ECM) structure with adequate mechanical property, but also allows the control of structure at the nano-, micro-and macro-scales that is beneficial for flexible tissue design and regeneration.In this study, PLA/HA/GO nanocomposites, from a series of DMF-dichloromethane suspensions consisted of PLA, HA and GO, were fabricated. The effect of the HA and GO addition agents on the morphologies and the structures of the resulting nanocomposites were investigated using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Diffraction (XRD), respectively. Furthermore, the MC3T3-E1 cells were cultured on the surfaces of the PLA/n-HA/GO membranes in order to explore their biocompatibility. As results, nanostructural GO and HA can be dispersed homogeneously in the nanocomposites and enhance the thermal stability of the PLA membrane. Under the technical parameters:the spun solution of 12 wt% of PLA, PLA/HA=(9/1 wt%), PLA/GO=(100/1,100/3. 20/1 wt%), the electrode distance of 15 cm and the voltage of 15 kV and the flow of 0.3 ml/h, the electrospun PLA/HA/GO nanofiber had the narrowest diameter of about 500～600 nm. In addition, after the cell growth, the MC3T3-E1 cells attached firmly on the PLA/HA/GO membranes and exhibited a favorable growth status. Therefore, the PLA/HA/GO nanocomposite membranes promoted cell seeding and cells ECM, also improved the biocompatibility of PLA material.