Research In The Protective Function Of Optimum Compatibility Radix Astragali And Kangfangchi In Acute Nephrotoxicity Rats

Since Belgian scholars reported that sufferers had taken Kangfangchi and Cortex magnoliae officinalis medicine for banting and suffered from nephrotoxicity in 1993,subsequently it was finded to be caused by aristolochic acid.So it was defined aristolochic acid nephrop athy(AAN). It has been more than decade to this day.There are many reports of clinical and experimental researches about clinical and pathologic characteristics and mechanism of AAN in the course. Kangfangchi belongs to Aristolochiaceae.In recent years,a great deal of domestic and foreign literature study nephrotoxicity of Kangfangchi and prevention and cure of AAN from various viewpoints.In order to make domestic and foreign scholars know Chinese herbal combination is available and scientific,author study Radix Astragali alleviate and detoxify acute nephrotoxicity made by Kangfangchi in microcosmic level.Objects:This experimentation studies Radix Astragali alleviate and detoxify acute nephrotoxicity made by Kangfangchi in cell level,riddles the optimal combination proportion of Radix Astragali and Kangfangch,testifies the significance and rationality of Chinese herbal combination in clinic.Methods:1,Choose healthy female wister rats,buy Kangfangchi from Zhaoqing Guangdong.2,Decoct respectively Kangfangchi and Radix Astragali,in succession,decoct together Radix Astragali and Kangfangchi in enactment proportion.Then filtrate,concentrate,desiccation and comminute respectively.3,Separate the experimental rats with randomization into16 groups,dose the rats with various proportional Radix Astragali and Kangfangchi.Mensurate the renal function and urine NAG andβ₂-MG of the rats,make the urine and blood routine assay,have the physiological and pathological examination of renal tissue after 14 days,Then analysis all the data.Results:To observe the actions and avoirdupois changes of the rats,to observe the urine routine assay of the rats,to observe the renal function of the rats,to observe the NAG andβ₂-MG in urine of the rats,to observe the renal tectology of the rats,all show that the combination of Radix Astragali and Kangfangchi ameliorate the renal function,Radix Astragali alleviate and detoxify acute nephrotoxicity made by Kangfangchi.Conclusions:1,Kangfangchi induce more nephrotoxic without Radix Astragali,pathologic characteristics of AAN is visible.The more dose of Kangfangchi,the more nephrotoxicity.2,It is lesser nephrotoxic that Kangfangchi combines Radix Astragali.This shows that Radix Astragali can alleviate and detoxify acute nephrotoxicity made by Kangfangchi.3,It is the most obvious detoxification when Radix Astragali combine Kangfangchi in proportion with 5:2.