Effects Of Posttransplantation Jiggling Force On The Experimental Autotransplantation Of Teeth In Vivo

Objective:The present study was performed to determine whether posttransplantation bucco-lingual(palatal) jiggling force could improve the healing state of autotransplantation of teeth and prevent the phenomenon of ankylosis. We want to offer a better method for the healing of autotransplantation of teeth in the future.Materials and methods:For this purpose,maxillary central incisors and lateral incisors(I1,I2)were extracted separately from each of the 6 adult male brood of dogs, then autotransplantation was performed by transferring from one side to the other side. The autotransplantation of teeth were immobilized for 2 weeks. 2 weeks later, as experimental group, we choose one central incisor and one lateral incisor from each dog's transplanted teeth to apply bucco-lingual(palatal) jiggling force 50g(2 weeks each direction). The others belong to the control group. We use perio test to measure the tooth mobility before and after applying jiggling force and give daily care to the transplanted teeth during that time. Then all animals were sacrificed and the dissected specimen were prepared for histological study. The decalcified section of all the specimen were amplified and taken photographs serially and act out the shape of the tooth root. The root resorption rate was defined as: area of root resorption/total root area including cementum×100%.Results:1.The mobility of teethThe results of measurement were dealt with SPSS 12.0 by Paired-Sample T. The data showed there was significant difference between before and after applying jiggling force in the experimental group(P=0.008<0.01). From the value, we may certify that after applying jiggling force, the mobility of the experimental group's teeth is bigger than before applying jiggling force in physiological range.The results of measurement were dealt with SPSS 12.0 by Independent Sample T Test. The data showed there was significant difference between the experimental group and the control group(P=0.003<0.01). From the value, we may certify that the mobility of the experimental group is bigger than the control group in physiological range.2.Radiological examinationWe discovered that periodontal spaces of both of the experimental group and the control group were wider than normal teeth and there was no periapical periodontitis seen in X-ray.3.Histological observation and analysis for resultsThe phenomenon of root resorption was discovered both in the experimental group and the control group. But the experimental group's degree was limit and the control group's was more serious. In the experimental group, it was discovered that the periodontal membrance space broadened,the blood vessel dilated in the periodontal membrane and the tight collagenous fiber arranged regularly on the experimental teeth; In the control group, the periodontal membrance space broadened in the majority, but decreased or lost in some area. The loosen collagenous fibers that were discovered arranged irregularly, disorganized around the root of tooth. The phenomena,such as the serious ankylosis, the hyalinization of periodontium, soakage of inflammation cell never occurred from the findings of the present experiment.4.Quantitative analysis of the rate of the root resorptionThe results of measurement were dealt with SPSS 12.0 by Independent Sample T Test. The data showed there was significant difference between the experimental group and the control group(P=0.028<0.05). From the value, we may certify that the rate of the root resorption of the experimental group is less than the control group.Conclusion:1. Jiggling force can increase the mobility of teeth and promote periodontal healing in transplanted teeth, which is a better way to prevent the phenomenon of ankylosis.2. Jiggling force can decrease the degree of root resorption in transplanted teeth.