The Effect Of Quantity Of Periodontium On Extracted Human Premolars For Orthodontic Treatment With Jiggling Force

Objective:The present study was designed to determine whether pre-extraction bucco-lingual(palato) jiggling force could affect the viability and quantity of periodontium on extracted human premolars for orthodontic treatment.We want to offer the definite foundation for autotransplantation with premolars for orthodontic treatment in the future.Methods:The subjects consisted of 10 males and 10 females who gave their informed consent. The bimaxillary unilateral first premolars were set as treated teeth randomly,accordingly first premolars of the other side as control teeth. Bucco-lingual(palato) jiggling force (100g) was applied to the treated teeth for a total of 6 weeks at intervals of 3 weeks for each direction.Then treated premolars were extracted then.Control premolars were extracted without the application of any force by the same operator before the extraction of treated teeth in each subject.And the time of extraction of all first premolars was recorded.After fixation and decalcification of the extracted premolars,each first premolar whose crown was removed was divided into two specimens bucco-lingually.The mesial specimens were prepared for a vertical-section histological study and stained with hematoxylin & eosin. And the distal specimens were divided into three segments:around the cervix,center and apex of the root,respectively,prepared for a cross-section histological study and stained with modified Mallory's trichrome method. Two pieces of series sections were get from different segments. All the sections of first premolars were examined by LM and taken photographs by digital camera of LM.From every vertical section,three segments were determined:apex,center and...