Establishment Of BMI Back Scar Model

Objective:To investigate the effects of the different trauma factors on hypertrophic scar formation in BMI back.Providing a accurate,practical,reproducible,and economical animal model for systematically studying scars.Methods:Full-thickness skin excision or 3-degree burns was used to create the lesions on the back of BMI intact skin.Full-thickness skin excision was performed in groupⅠ,and 3-degree burn injury was inflicted in groupⅡ,to see whether wounds would heal with hypertrophic scars.Samples were harvested at several time points post wound.Clinical appearanee of BMI wounds was observed.The section of tissue was stained with HE.Scars developed were examined morphologically by light microscopy and biochemically by typeⅠ,Ⅲcollagen immunohistochemical staining.Analytic result of computer image.Results:Full-thickness skin excision were normal healing without scar.The mean value of wound healing time were 20d,3-degree burns wound with some clinical and histologic on BMI back are similar to that in human hypertrophic scar.The mean value of wound healing time were 35d,respectively,which have statistical differecnce。Analytic result of computer image:In clinicopathological,typeⅠcollagen was about 74%;typeⅢcollagen accounted for about 26%.In the samples from 7-9 weeks,the contents of typeⅠcollagen were(74.52±0.47)%,(74.43±0.53)%,(74.69±0.63)%, respectively;the contents of typeⅢcollagen were(25.48±0.47)%,(25.57±0.53)%, (25.31±0.63)%,respectively,which had insignificant difference(p＞0.05)Conclusion:It is shown that it is possible to develop experimental hypertrophic scars in Banna minipig inbred line by 3-degree burns.Those scars are with morphological and biochemical properties similar to those of human hypertrophic scars.Therefore this model is a practical,reproducible,and economical animal model for systematically studying hypertrophic scars,although improvements are needed to increase yield.