| Background and the aim Diabetes mellitus （DM） is the third-largestnon-communicable diseases following malignant tumors, cardiovascular andcerebrovascular disease. The incidence in the population is increasing and it seriouslyendangers people’s health and lives. PKB is a serine/threonine protein kinase, andprevious studies have shown that it participates in the body regulate glucose and lipidmetabolism, promote cell survival, and inhibit apoptosis. The study intends to establisha new diabetes model with Banna minipig by high-sucrose/high-fat diet and mild STZtreatment, and then observe the pathological changes of liver, kidney and skeletalmuscle by light microscopy and electronic microscopy. The levels of PKB mRNA,PKB and p-PKBSer473protein are detected in these tissues. The aim of the study toestablish a new diabetes model with Banna minipig and explore the role of PKB in thedisorder of glucose metabolism and insulin resistance.Materials and methords Eight male Chinese Banna minpigs were weighted andrandomly into two groups, including control group and treatment group.Control groupfed with a control diet （CD） for12months, and treatment group fed withhigh-sucrose/high-fat （HSFD） and treated with STZ. After fasting, the blood sampleswere collected, and the content glucose, insulin, total cholesterol （TC） and triglyceride（TG） in blood were detected. Finally before animals were sacrificed, intravenousglucose tolerance test （IVGTT）, the calculation of the glucose tolerance index andinsulin sensitivity index were analyzed. And then, the Banna minipigs were sacrificedand the tissue of pancreas, liver, kidney and skeletal muscle were detected. Thepathological changes of liver, pancreas, kidney and skeletal muscle by light microscopyand electron microscopy. The mRNA levels of PKB were detected by teal time PCR,the protein level of PKB and p-PKBSer473were measured by immunohistochemistry andwestern blot in the pancreas, liver, kidney and skeletal muscle. Kidney cell apoptosis were examined by TUNEL.Results A new diabetes model of Banna Chinese minipigs were establish by highsucrose/high fat/STZ treated, and accompanied by the lesion of the pancreas, liver,renal and skeletal muscle. PKB mRNA level in the skeletal muscle was significantlydecreased, was significantly increased in the pancreas and liver. The total PKB proteinlevel, showed no significant difference （P>0.05） between the HSFD group and the CDgroup.The p-PKBSer473protein level in the HSFD group was less than the CD group（P<0.05）. The number of renal cell apoptosis were increased.Conclusion High sucrose/high fat/STZ-induced Banna Chinese minipigsdiabetes models successfully.PKBmRNA expression was significantly upregulated inliver and kidney tissue, was significantly lowered in skeletal muscle tissue.Total PKBprotein expression of the two groups was no signicant difference, p-PKBSer473expression was significantly reduced. |
