| The effect of hydroxyapatite nano-particles on cancer cells is a kind of biological effect of inorganic nano-particles, and it had been studied by Bomedical Materials and Engineering Research Center of Wuhan University of Technology since many years before. On the basis of the former researeh, the author selected "the effect of HAP nano-particles on the mitcochondria of liver rats" as his main research content in the subject. In this study, we have studied HAP nano-particles effect of mitochondria from the perspective of organelles, tested SDH activity of mitochondria, and explored the effect of HAP nano-particles on the mitcochondria. Therefore this study aims to provide the theoretical foundation for further researching inhibitive effect on cancer cell.Liver mitochondria of rat were isolated and extracted by traditional methods, we obtained active mitochondria by the test of SDH which is a key marker of mitochondria; we investigated the condition of SDH test of mitochondria; last, we put different concentrations of HAP nano-particles to directly treat mitochondria with negative control, then conclusions are made:1. The SDH activity of mitochondria was 7.376±0.910 u/mg protain, it fit to our experimental request;2. The SDH activity of mitochondria was higher at 37℃but its activity disappeared after two days, and the same as in two months at -70℃;3. HAP nano-particles inhibit the SDH activity of mitochondria, and became lower and faster compared with negative control depend on time and dose.We bettered the test of mitochondrial activity by SDH kit, which made test more quickly, conveniently and cut down its cost. Moreover we put HAP nano-particles to mitochondria directly, which based on the traditional cell level research.
|
PDF Full Text Request