| ObjectiveTo detect and compare the expression of human programmed cell death 4 and surviving among normal foreskin,condyloma acuminatum(CA),bowenoid papulosis(BP),Bowen's disease(BD)and squamous cell carcinoma(SCC);And to explore the significance of the human programmed cell death 4 and Survivin in normal tissue,benign tumor and malignant carcinoma;To predict the prognosis of bowenoid papulosis.·Methods1.All cases diagnosed as CA,BE BD or SCC in the last nine years in our hospital were collected and the case of each disease were 30,28,10,11 respectively.10 normal foreskin specimens were fixed by formaldehyde and embedded by paraffin.2.The expression of PDCD4 and Survivin in keratinocytes,was detected by immunohistochemistry technique.3.The expression of PDCD4 and Survivin were compared among normal skin, CA,BP,BD and SCC.Results1.Location and intensity of immunohistochemical stain(PDCD4)Positive expression was located mainly in the nuclear,partially in the plasma.Normal foreskin specimens were all positive;in CA,most cells are positive;in BP and BD,less positive cells with color diluter were observed:in SCC,almost all the cells are negative.2.Positive rates of all groups.(PDCD4):The positive rates in normal foreskin,CA,BP,BD,and SCC were 100%,76.7%,46.4%,30%,0%,respectively。3.Comparison of positive expression intensity among all groups(PDCD4). There was no significant statistic difference in the expression of PDCD4 between normal foreskin and CA(P＞0.05),while there was significant statistic difference between normal foreskin and the other groups(P＜0.05). Also,the expression of PDCD4 was no significant different between CA and BP,BD(P＞0.05).the expression of PDCD4 was significant different between CA and SCC(P＜0.05).There was no significant statistic different between BP,BD,SCC(P＞0.05).4.Location and intensity of immunohistochemical stain(Survivin).Positive expression was located mainly in the plasma and the nuclear. Normal foreskin specimens were all negative;in CA,only a few cells were dyed with light color and most of them were vacuole cells;In BP and BD, more positive cells with deeper color were observed and most of them were atypical cells in every layer of the epidermis;in SCC,positive-dyed cells were skattered around and the color was deeper than that in BP and BD.5.Positive rates of all groups(Survivin).The positive rates in normal foreskin,CA,BP,BD and SCC were 0%,53.3 %,64.3%,70%and 81.8%,respectively。6.Comparison of positive expression intensity among all groupsThere was no significant statistic'difference in the expression of Survivin between normal foreskin and CA,BP,(P＞0.05).There was significant statistic difference between normal foreskin and BD,SCC(P＜0.05).Also, the expression of suvivin was no significant difference between CA and BP, BD(P＞0.05).the expression of suvivin was significant difference between CA and SCC(P＜0.05).There was no significant statistic difference between BP and BD,SCC(P＞0.05).7.The positive rate of PDCD4 in BP is lower than CA,and higher than it in SCC.The positive rate of Survivin in BP is higher than CA and lower in SCC.Conclusions1.The expression of PDCD4 in normal skin,benign tumor and malignant carcinoma decrease gradually,which suggests that the expression of PDCD4 plays an important role in the inhibition of cellular proliferation.2.The expression of Survivin in normal skin,benign tumor and malignant carcinoma go rise up gradually,which suggests that the expression of survivin plays an important role in both cell proliferation and immortalization.3.The positive rate of PDCD4 in bowenoid papulosis was lower than that of condyloma acuminatum,and higher than that of Carcinoma squamous cell;the positive rate of Survivin in bowenoid papulosis was higher than its in condyloma acuminatum,and lower than its in Carcinoma squamous cell.All these were hint that the malignant degree of bowenoid papulosis was between carcinoma squamous cell and non-malignant hyperplastic disease.It has the tendercy to develop to the carcinoma.So,it should be observe and long-term follow-up. |
