The Experimental Research On The Effects Of Bu Yang Huan Wu Decoction For β-Amyloid Protein Precursor And Apoptosis Protein Of AD Rats

Starting from the theories of AD,the hypothesis of cell apoptosis and the hypothesis ofβ-amyloid protein cascades,and based on the metabolic pathway of Aβthat participates in AD,this experiment employed Aβ_1-40to inject the Hippocampus to induce neuronal damage in brain and to copy the AD Rat Subject.By the curative target ofβ-amyloid protein deposition and neuronal damage pathological changes and cell apoptosis,we adopted Morris water maze and neuropathology to observe the changes of ethology and brain histomorphology of AD Rat;adopted immunohistochemical approach to detect the expression of Aβ_1-40,β-APP,bcl-2,bax,caspase-3 in Hippocampus of rat. Then we would from the ethology and molecule levels study the therapeutic efficacy and amynology mechanism of Buyang Huanwu Decoction for AD rat induced by Aβto provide experimental data for clinical application.The results of the study indicated that Buyang Huanwu Decoction had greatly lessened the memorizing dificulties of AD Rat Subject brought about by Aβ;greatly lowered the highly increased Aβdeposition in Hippocampus and brain mantle;greatly lessened neuronal damage caused by Aβ;stepped up the expression of bcl-2 in Hippocampus of AD rat model;then could lower high expression of bax and caspase-3 in Hippocampus;finally stepped up bcl-2/bax'ratio,Repress cell apoptosis.Buyang Huanwu Decoction was likely to,by the regulation of stepping up the expression of bcl-2,of lowering the high expression of Bax and caspase-3 and of stepping up bcl-2/bax'ratio,restrain cell apoptosis,interfere with the generation and metabolism ofβ-APP and control appropriately the cell apoptosis and mitochondrion damage caused by over deposition of Aβ. Accordingly,the decoction lessened the brain neuronal damage effectively caused by Aβtoxic effects and naturally relieved the memorizing difficulties and prevented AD.