The Experimental Study On The Effects Of Bu Yang Huan Wu Decoction For β-Amyloid Precursor Protein And Cytokine Of AD Rats

On the beginning of the theories of AD,which were the hypothesis of nerves immune inflammation and the hypothesis ofβ-amyloid protein cascades, and based on that the metabolic pathway of Aβparticipated in AD,The topic adopted Aβ_1-40to inject the Hippocampus to induce neuronal damage in brain,to copy the AD rat model.By the curative target ofβ-amyloid protein deposition and neuronal damage pathological changes,we used Morris water maze and neuropathology to observe the changes of ethology and brain histomorphology of AD rat;used radioimmunity method to determine the levels of Aβ,IL-2 and TNF-αin serum and Hippocampus of rat;and used immunohistochemical method to detect the expression of Aβ_1-40,β-APP in Hippocampus of rat.Then we discussed the therapeutic efficacy and amynology mechanism of Buyang Huanwu Decoction for AD rat induced by Aβfrom the ethology,cell and molecule levels,therefore provided the experimental evidences for clinical application.The results of the study indicated that Buyang Huanwu Decoction had obvious improved effects for dysmnesy of AD rat model induced by Aβ;could lower the obviously increased Aβdeposition markedly in Hippocampus and brain mantle;and improved neuronal damage caused by Aβ,and improved nerves fibronolysis damage markedly,lessened inflammatory reaction in brain; so could lower obviously the abnormal high expression ofβ-APP protein in Hippocampus of AD rat caused by Aβ;lowered high levels of Aβ,IL-2 and TNF-αin serum and Hippocampus of AD rat model.Buyang Huanwu Decoction might regulate immunity inflammatory cytokine IL-2 and TNF-αof blood and brain and interfere in the generation and metabolism ofβ-APP,control reasonably the immune inflammation cascade reaction caused by Aβdeposition.Thereby lessened brain neuronal damage effectually caused by Aβtoxic action,and improved the dysmnesy.Then achieved the effect of prevention and cure of AD.