Expression Of Protein Kinase Cα, βⅡ, δ In The Rat Ratina During The Development Process

Objective:To investigate the exprepression and distribution of protein kinase Cα,βⅡ,δin the retinal during the development of poatnatal hamster.Methods: Real time RT-PCR method was used to detect the expression of mRNA;Immunocytochemical menthod was used to detect peotein.Results : The rusult of Real time RT-PCR showed that PKCα,βⅡmRNA was continual rise before 14th day of poatnatal development and then reduced till the lowest point in the adulthood. PKCδwas highly express in the early period of poatnatal development.The result of of spectrodensitometry was match to the result of Real time RT-PCR . In the first day(P0), the expression of PKCα,βⅡwas poor , the expression of PKCδwas found in neuroblastic layer and ganglion cell layer.With the development, at the seventh day, PKCαwas pronoounced in the external plexiform layer and inner nuclear layer; PKCβⅡwas pronoounced in the inner nuclear layer,inner plexiform layer, external plexiform layer and ganglion cell layer; PKCδwas pronoounced in the external limiting membrane and cone and rod layer.At the 14th day, isozymes were more localized than before. With mature the three isoenyme distribute invariably.Conclution: Protein kinase Cα,βⅡ,δwere expressed regularly in the whole process of rat retina development. It is suggest that the three isoenzyme is closely related to the early development of ratina in the postnatal hamster;The changed expression of PKCα,βⅡ,δis suggested as: each isoenzyme may have different roles in processing of rat ratinal development. PKCαmay be important to retinal rod-bipolar cells development; PKCβⅡmay be important to ratinal ganglion cells; PKCδmay participate the process of differentiation and Apoptosis of Primitive neuroblastic cells and Primitive ganglion cells in the early period .