| As a common type of encephalic tumor, meningioma accounts for about 15%-20%[1] of all encephalic tumors. It is mainly derived from arachnoid meningothelial cells tumors. The key biological characteristics of encephaloma are local invasion and early tumor cells invasion into its surrounding encephalic cells. The invasion behavior of meningioma is one of the reasons of aftertreatment recrudesce, it is not only affecting the patients'prognosis, but also leads to the high rate of disability and death. According to some research, the decreasing conglutination between, and the increasing movement of tumor cells are the main symbols of tumor invasions.Tight junctions (TJs), which lies in between the two neighbouring top epithelium cells, are the main junction form between epithelium cells, endothelium cells, and the top cells of the inter-myelinated cells' lateral membrane. TJs has many functions, including closing cell spaces, maintaining cell polarity and conglutination, adjusting cell growth and polarization, etc.Because its special structure and function, TJs exception is currently considered to be the prerequisite of the tumor cells invasion. There is three different type of necessary protein of membrane, claudins, occludins and JAM, which are located at TJs. PKC is the important factor which controls the TJs formation and adjusting, and plays a important role of maintaining the TJs'structure and function. Until now, it is not very clear about the TJs'function mechanism in the process of meningioma invasion, and there are seldom reports of the relativity research between claudin-6, occluding, PKCθand meningioma.In this experiment, the expression of claudin-6, occludin, and PKCθin 29 non-invasion and 29 invasion meningioma tissue is tested by using immunohistochemistry method (SP), with the goal of analyzing the relationship between the expression of claudin-6, occludin, and PKCθin the non-invasion and invasion meningioma against its biological indicators, so as to further discuss the function of claudin-6,occludin,PKCθin invasion and development of meningioma. Results are as following:1. The positive rate of claudin-6 was 10.3% (3/29) in invasion meningioma tissue and 65.5% (19/29) in non-invasion meningioma tissue. The difference is significant (P<0.05). The expression of claudin-6 was not dependent on the age and sex of meningioma patients.(P>0.05)2. The positive rate of occludin was 48.3% (14/29) in invasion meningioma tissue and 72.8% (21/29) in non-invasion meningioma tissue. The difference is not significant (P>0.05). The expression of occludin was not dependent on the age and sex of meningioma patients.(P>0.05)3. The positive rate of PKCθwas 34.5% (10/29) in invasion meningioma tissue and 82.8% (24/29) in non-invasion meningioma tissue. The difference is significant (P<0.05). The expression of PKCθwas not dependent on the age and sex of meningioma patients.(P>0.05)4. Each two correlation analysis in the expressions of claudin-6, occludin and PKCθin meningioma tissue did not show any significant relations between occluding and claudin-6, PKCθprotein (P>0.05) , but show relations between PKCθand claudin-6 protein. (P<0.05)The above results suggest that the lower expression of claudin-6 in invasion meningioma tissue probably affects occurrence and invasion of the meningioma by changing the structure and function of TJs; and the abnormal activation of PKCθin the invasion meningioma may has a certain adjustive effect on the structure and function of TJs. The claudin-6 and PKCθcould be useful as a reference indicator to estimate the meningioma invasion.
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