The Study Of The Growth Suppression Of Cutaneous Squamous Ccll Carcinoma Cells By Adenovirus-pkcδ Infection

Objective: The purpose of this study was to investigate the role of PKCδ incutaneous squamous cell carcinoma. The study observed the effect of PKCδ on SCC13cells proliferation through the infection of Adenovirus-PKCδ in vitro. In vivo, the studyshowed the ability of PKCδ infected-SCC13cells in tumorigenicity in NOD/SCIDmice.Methods:1. Ad-PKCδ infected the SCC13cells. Western blot method was usedto detected the expression of PKCδ.~3H-thymidine incorporation assay was used todetected the proliferation activity of the SCC13cells.2.Ad-PKCδ-treated and control groups of SCC13cells were implanted intoNOD/SCID mice. Comparative capability of the tumorigenicity was observed. Thediameter of the tomors was measured and recorded, Hematoxylin-eosin staining wasused observe the histomorphology of tumors under the light microscopy.Results:1. The proliferation of SCC13cells was obviously inhibited after infectedwith Ad-PKCδ. p<0.05, compare to control group, n=3.2. Tumors were successfully implanted in both of control and Ad-PKCδ-treatedgroups. Tumors formed in all mice. We found sizes of tumors in Ad-PKCδ-treatedgroup were smaller than the sizes of tumors in control group through measurement ofthe diameters.Conclusion: In summary, the study revealed that PKCδ significantly inhibitedSCC13cells proliferation in vitro, and dramatically suppressed tumor growth inNOD/SCID mice in vivo. These findings indicate that PKCδ gene mediated byrecombinant adenovirus may have an antitumor activity against cutaneous squamouscell carcinoma cell, suggesting its potential clinieal applieation and provides anelementary basis for gene therapy against cutaneous squamous cell carcinoma.