Study Of Zinc On Glycemia And Prevent The Early Impairment Of Nerve In Diabetic Rats

Objective: To observe the effects of zinc on glycemia in diabetic rats. To observe the electrophysiological changes of sciatic nerve in early diabetic rats.Methods: Diabetic SD rats model were induced with streptozotocin(STZ) 55mg/kg, to observe the incidence and survival. To observe the effects of zinc on glycemia in diabetic rats. 10 rats were selected randomly for normal group form 80 SD male rats, and other 70 rats were injected peritoneal with 55mg/kg STZ, and 59 rats became diabetic rats. They were randomly divided into five groups according to zinc lever: model, low dose, medium dose, high dose and acarbose group. Normal and model group were given distilled water through tube feeding without any treatment everyday. Low dose group was treated with zinc 7.5mg/kg·bw, and medium group 15mg/kg·bw,high group 30mg/kg·bw everyday, Acarbose(0.01g/kg·bw) everyday. The treatment went on 30days.we detected FBG through trail vein in the fifteenth and thirtyth day, and confirm the adequate dosage of zinc. The electrophysiological changes of sciatic nerve in diabetic rats happened after zinc treatment, The administration was ceased on the 2th week, and the blood glucose and body mass of rats were inspected. The sciatic nerve in rats of all anesthetized groups were isolated and exposed. The latency, amplitude of wave and the nerve conduction velocity of sensory nerve action potential were detected respectively. Difference in data were compared with analysis of variance, and differences between each two groups were compared with Scheffe test.Results: 59 rats became diabetic rats after injected STZ 55mg/kg·bw, and the model rats was 84.3%, the livability was 100%. Compared with model group 5weeks later, FBG of high dose and medium dose group was significantly decreased (P<0.01).The nerve conduction velocity and amplitude wave of sensory nerve action potential in the diabetic model group were significantly reduced than in the normal control group. Zinc therapy could effectively reverse these changes, and the conduction velocity of sensory nerve action potential of sciatic nerve increased to 46.25m/s, the wave amplitude ascended to 586.98uv, and the latency was shortened to 1.30ms compared with diabetic model group, there were remarkable differences.Conclusions: To induce SD diabetic rats with 55mg/kg of STZ had high model rats and livability. Zinc can decrease FBC significantly in diabetic model rats, and the adequate dose was 30mg/kg·bw and 15mg/kg·bw.There were functional deficits of sensory nerve in early stage of diabete(2weeks), the cause of which is hyperglycemia, and the impairment of nerve can be effectively controlled by zinc.