The Experimental Study On Acupuncturing The Upper2/5 Of Motor Zone To The Repair Of Sciatic Nerve Injury In Rats

Objective: Observing the influence of the head acupuncture to the regeneration and repair of the sciatic nerve injury in rats at different time, unveils the active mechanism of the regeneration of the sciatic nerve injury in rats at different time, offers the theoretical basement clinically.Methods: The Wistar male rats are the testers in this test. Adopts the model of peripheral nerve injury by nipping the sciatic nerve, makes these 180 rats into six groups: (1) the group of acupuncturing the upper 2/5 of motor zone (the head acupuncture group);(2) the group of acupuncturing the upper 2/5 of motor zone+ the local (the head and body acupuncture group);(3) the local acupuncture group (the body group);(4) the drug group;(5)the model group;(6)the pseudo operation group. Every group is divided into three time segments (7d, 14d, and 28d). Observes the change in morphologic of the injured nerve and the corresponding spinal segment with HE staining;looks into the recovery condition of the function dynamically in electrophysiology;examines the expression of the IGF and FGF in the injured sciatic nerve cells with immunohistochemistry.Results:1.The MNCV increases at different extents in these acupuncture groups and the drug group, the comparison between treatment groups showed significant difference (P<0.05) .2. Not only the head acupuncture and body acupuncture can relieve the pathological changes degree of nerve fiber resulted in peripheral nerve injury, accelerate the regeneration of the nerves, and the effect is superior to drug group. The combination of the head acupuncture and body acupuncture is second to none.3. The expression level of IGF and FGF of head acupuncture group is significant. The comparison between treatment groups showed significant difference (P<0.01) .Conclusions:1. The head acupuncture can promote the recovery of the motor nerve conductive velocity (MNCV) of the sciatic nerve injury in rats.2. The head acupuncture can relieve the swell and the denaturalization of the nerves and the nerve cells which are located in the corresponding spinal segment, cut down the death of the nerve cells and accelerate the regeneration of the axon after the sciatic nerve of the rat is injured.3. The head acupuncture can restrain the decreasing of the expression level of IGF and FGF in the nerve cells of rats whose sciatic nerves are injured, makes them recover, and anymore protects the nerve cells, accelerates the regeneration of the nerves.4. The combination of the head acupuncture and body acupuncture is second to none. As to promoting the regeneration and repair of nerve injury, it can do better.