The Study Of Different Dose's Low Molecular Weight Heparin(LMWH) On Vascular Morphological Changes Of Acute DVT In Rats

Objective To observe the effect of different dose,s Low molecular weight heparin( LMWH) on morphological changes of vein wall after differenct periods of deep vein thrombosis (DVT) in rats. Methods Firstly,72 sprague-dawley (SD) rats were randomly divided intoⅠ,Ⅱ,Ⅲ,Ⅳperiods on 1st,3rd,7th,14th day following thrombosis respectively, there were 18 SD rats in every period.Then the 18 SD rats in each period were separated to 4 group. Low dose's Low molecular weight heparin( LMWH) group (700u/kg,1t/24h, n=6.),high dose's Low molecular weight heparin(LMWH) group(700u/kg, 1t/12h, n=6.),thrombosis group(0,000u/kg, n=6). In each group, to observe the remain quantity of endothelial cells and the expression areas of collagen by dyeing. Results 2 SD rats in thrombosis group were died inⅡ,Ⅲperiod respectively. 4 SD rats in high dose's Low molecular weight heparin( LMWH) group were died inⅠ,Ⅲ,Ⅳperiod in thrombolytic therapy. InⅠperiod, the quantity of endothelial cells in low,high dose's LMWH group have significant difference with thrombosis group respectively (P<0.05). The difference exisited in interclass as well(P<0.05). InⅠperiod, the expression areas of collagen in low,high dose's LMWH group have significant difference with thrombosis group respectively (P<0.05); InⅡperiod, the quantity of endothelial cells in low,high dose's LMWH group have significant difference with thrombosis group respectively (P<0.05) ,The difference do not exisited in interclass (P>0.05);InⅢ,Ⅳperiods,the quantity of endothelial cells in low,high dose's LMWH group haven,t significant difference with thrombosis group respectively(P>0.05). InⅡ,Ⅲperiod, the expression areas of collagen in low,high dose's LMWH group have significant difference with thrombosis group respectively (P<0.05), The difference exisited in interclass as well(P<0.05)。InⅣperiod, The expression areas of collagen in low,high dose's LMWH group haven,t significant difference with thrombosis group respectively (P<0.05). Conclusions Different dose,s LMWH have protective effect on the quantity of endothelial cells during 3 days after thrombosis, the effect of high dose's LMWH is better; Different dose,s LMWH haven,t protective on the quantity of endothelial cells after 7 days following thrombosis,but they have inhibitary effect on the expression areas of collagen in every period, the effect of high dose's LMWH is better.