The Clinical Value Of Detection Of Tumor Markers By Protein Chip Technology For Breast Cancer

Objective:1.The research is to detect tumor markers(TM)in serum of the patients with breast cancer,the patients with benign breast disease and control groups by the multi-tumor marker protein biochip detective system,then choose tumor markers which have clinical value for breast cancer,analyze the diagnosis value and the cause of abnormity; 2.Tumor markers in serum of the patients with breast cancer are detected by the multi-tumor marker protein biochip detective system,then analyze the relations between diameter of primary tumor,axillary lymph node metastases(ALNM)and tumor markers,and investigate the clinical value of tumor markers about the severity of breast cancer;3.Tumor markers of the patients with breast cancer both before and 3 months after operation and the patients with recurrent breast cancer are detected by the multi-tumor marker protein biochip detective system,then investigate the prognostic value of tumor markers in breast cancer;4.We assess the clinical value of protein chip technology by applying to clinic.Method:By the multi-tumor marker protein biochip diagnostic system,we detected 12 kinds of tumor markers,which included carbohydrate antigen 199(CA199),alphafetoprotein(AFP), neuron-specific enolase(NSE),free prostate specific antigen(f-PSA), carcinoembryonic antigen(CEA),prostate specific anitgen(PSA), carbohydrate antigen 242(CA242),carbohydrate antigen 125(CA125), carbohydrate antigen 153(CA153),FER(Ferritin),human growth hormone(HGH),and human chorionic gonadotropin(HCG)in 90 cases of breast cancer,60 cases of benign breast disease and 100 cases of healthy individuals as control groups;First,we get blood samples from the patients,then centrifuge the samples;We dilute the standard samples before testing;Put the samples into the different compartments of protein chip;Incubate and surge the protein chip,then wash it;Put the reagent into the compartments of protein chip;Peel off the top of protein chip and wash it;Put testing reagent into the compartments,then gain the results by the multi-tumor marker protein biochip detective system.SPSS version 13.0 was used for statistical analysis;Use Chi-square test for enumeration count data;Use partition ofχ2 method for comparing rates of three groups;Use t-test for measurement data;use SNK-q test for comparing means of three groups;Assess the clinical value of tumor markers for breast cancer by logistic regression and establishing logistic regression equation;The area under the ROC curve(AUC)of CEA,FER, CA125,CA153 from logistic regression results were compared.Results:â‘ The serum levels of CEA,FER,CA125,CA153 in the patients with breast cancer were significantly higher than that in control groups and the patients with benign breast disease(P＜0.01);There was no significant difference between the patients with benign breast disease and control groups(P＞0.05);The serum levels of CA199,NSE,CA242, HCG,AFP,f-PSA,PSA,HGH had little significant difference between the patients with breast cancer,the patients with benign breast disease and control groups(P＞0.05);The logistic regression equation of groups of breast cancer and control groups is P₁=1/1+e^{-(-1.29+2.25×CEA+3.12×CA125+10.59×CA153)};In cancer-control group,the AUC of combination of CEA,FER, CA125 and CA153 was larger than any AUC of CEA,FER,CA125 or CA153 alone(P=0.000),the AUC of CA153 was larger than AUC of CEA,FER,CA125(P=0.000);â‘¡The serum levels and positive rates of the CEA,CA125,FER,CA153 in the patients with axillary lymph node metastases(ALNM)were significantly higher than that in the patients without ALNM(P＜0.01);The logistic regression equation of groups with ALNM and groups without ALNM is P₂=1/1+e^{-(-2.27+2.05×CEA+1.94×FER+1.06×CA125+1.84×CA153)};In groups with ALNM and groups without ALNM,the AUC of combination of CEA,FER,CA125 and CA153 was larger than any AUC of CEA,FER,CA125 or CA153 alone(P=0.000),the AUC of FER was larger than AUC of CEA,FER,CA125(P=0.000).The serum levels of the CEA,CA125,FER,CA153 in the patients with the diameter of primary tumor over 3 centimeters were significantly higher than that in patients with the diameter of primary tumor below 3 centimeters(P＜0.01).â‘¢The serum levels of CEA,FER,CA125 and CA153 was significantly lower after operation than that before operation in the patients with breast cancer(P＜0.01);There was no significant difference between the patients 3 months after operation and control groups(P＞0.05);The serum levels of CEA,FER,CA125,CA153 in the patients with recurrent breast cancer were significantly higher than that in the patients without recurrent breast cancer(P＜0.01);The logistic regression equation of groups with recurrent breast cancer and groups without recurrent breast cancer is P₃=1/1+e^{-(-21.00+11.50×CEA+20.31×CA125+21.70×CA153)},In groups with recurrent breast cancer and groups without recurrent breast cancer,the AUC of combination of CEA,FER,CA125 and CA153 was larger than any AUC of CEA,FER,CA125 or CA153 alone(P=0.000),the AUC of CA153 was larger than AUC of CEA,FER,CA125(P=0.000).Conclusion:â‘ The application of C-12 multi-tumor marker protein biochip diagnostic system in diagnosis,observing recruuent and metasetases of breast cancer is valuable;â‘¡CEA,FER,CA125 and CA153 can be regarded as valuable tumor markers for breast cancer; Combining detection of CA125,CA153,CEA,FER could increase the diagnostic sensitivity of breast cancer;â‘¢The serum levels of CEA,FER, CA125 and CA153 are risk factors of ALNM,and related with the diameter of primary tumor;â‘£Detection of serum CA153,CA125,FER and CEA levels is valuable for prognosis in the patients with breast cancer.⑤Combined detection of CEA,FER,CA125 and CA153 is valueable for judging ALNM,evaluation the effect of operation,observing recurrence and metastasis and evaluation of the progress and prognosis of breast cancer;CEA,FER,CA125 and CA153 is a better combination of tumor markers for breast cancer;â‘¥CA199,NSE,CA242,HCG,AFP,f-PSA, PSA,HGH have little value for breast cancer;⑦As an advanced statistical method,logistic regression can improve the diagnostic sensitivity and specificity.