| ObjectiveTo investigate the relationship between the level of monoamine neurotransmitters and the change of ethology after nucleus accumbence lesioned in morphine-addicted rats,and observe the imageological changes in brain regin of morphine-addicted rats before and after stereotaxic lesion.MethodsAn acute morphine-addicted rat mode was made with morphine by abdominal cavity injection. The interval of the injection is 12 hours. The dose of injection was added by daily from 5 mg/kg to 90 mg/kg within 10 days. Before and after stereotaxic lesion, the level of monoamine neurotransmitters in different brain regions was detected by a fluorescence detector (high performance liuid chromatography). The relationship between the monoamine neurotransmitters and ethological changes was analyzed after nucleus accumbence lesioned in the experiment rats.Results(1) There was a high successful rate in making acute morphine-addicted rats, and remarkable ethological changes in morphine-addicted rats before and after stereotaxic lesion.(2)There were significant difference in ethological changes between nucleus accumbens-lesioned group and nucleus accumbens pseudo-lesioned group.(3)After nucleus accumbence lesioned, the abstinence syndrome could lessen. The levels of DA, NE, 5-HT in the regions of prefrontal cortex, hippocampus and ventral tegmental area were obvious changed compared with the unlesioned group in morphine-addicted rats (P<0.01).(4) Before and after lesion, the imageology of the morphine-addicted rats'brain are different too, the brain edma of the nucleus accumbence region were evidently.Conclusions(1)There may have some relationships between changes of ethology and the levels of monoamine neurotransmitters in the morphine-addicted rat.(2)Before and after lesion, the imageology of the morphine-addicted rats'brain are different too,the brain edma of the nucleus accumbence region were evidently. |
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