The Study Of Total Flavonoids Of Murraya Paniculata Leaves On The Effect Of Decreasing Blood Glucose

Diabetes morbidity and mortality have been increasing gradually currently. And it has become the third disease endangering human health after cancer and cardiovascular disease. Although the incidence of diabetes is not high in China compared with the developed countries, the absolute number of patients is the biggest in the world for the large population. Therefore, searching for safe and effective drugs used to prevention and cure Diabetes has become a focus of modern medicine. Currently, the oral hypoglycemic agents used for the T2DM includes: sulfonylurea derivatives, guanidine derivatives, glucosidase inhibitor, euglycemic agent and so on. But oral hypoglycemic drugs have many adverse reactions and toxic effect, so it is urgent to find a better drug in Chinese traditional medicine.The latest research on anti-diabetic monomer composition of traditional Chinese medicine mainly concentrated on alkaloids, flavonoids, polysaccharides, saponin and terpene. Flavonoids are widespread in nature. They have a wide variety and high-pharmacological activity. Many researches have proved that flavonoids could treatment diabetes and complications significantly, so it is important to study flavonoids'active ingredients to control diabetes and its complications. The leaves of Murraya paniculata is the main raw material of curry powder. Modern study have found that something extracted from the leaves of Murraya paniculata had the effect on hypoglycemic. Nowadays, many countries in the United States and European are studying the value of Murraya paniculata. Total flavonoids of Murraya paniculata leaves (TFMP) were extracted from the leaves of Murraya paniculata. The content of flavonoid has reached 92 percent or above. In order to explore TFMP as an effective anti-diabetes drug, we studied the effects of TFMP on experimental hyperglycemia of mice and rats. Then we can provide scientific basis for the further development and utilization of TFMP.TFMP were given to the normal mice, on 10th day epinephrine was administered through subcutaneous injection or glucose was administered through intraperitoneal injection in the fasting state to establish a mouse model of acute hyperglycemia, observed the blood glucose of the experimental animals how to change at different time after administering the TFMP through ig. Rats were randomly divided into normal group and model group .The normal group rats were fed by distilled water and the model group rats were fed by highly fatted food for 10 days and then injected by small dose of STZ30 mg/kg one time. The T2DM Rats were divided into TFMP17.5, 35, 70 mg/kg group, metformin group and diabetes model group by FBG and body weight. All animals were assayed the values of fasting blood glucose (FBG) every week, then assayed FBG, Ins, C-peptide, IL-6, TNF-α, IL-1β, TC, TG, HDL-c, LDL-c, MDA content and SOD activity in the blood after four weeks, assayed glycogen content in the rat liver and fixed pancreas by 10% formalin to do immunohistochemical analysis. The results have showed that:1, FBG has be increased significantly in model group after injected epinephrine; the 100 mg/kg dose of TFMP decreased FBG significantly (P<0.05) in 60 and 90min compared with normal group. We had a conclusion that TFMP could improve the hyperglycemia caused by epinephrine. 2, In the glucose tolerance test of the normal mice, FBG of model mice were increased significantly after injected glucose 30 ~ 120 min; the FBG of TFMP70mg/kg, 100mg/kg group were lower in the 60 min and 120 min after injected glucose, but there was no statistical significance. We had a conclusion that TFMP could improve glucose tolerance in normal mice, but the effect is not significant.3, In the model of hyperglycemia rats induced by high fat diet with low-dose STZ, TFMP 35mg/kg, 70mg/kg have decreased FBG significantly from the 3rd to the 4th week compared with the control group ( P <0.01 or P <0.05), the liver glycogen content were significantly increased after administrated four weeks (P <0.05), The results have suggested that TFMP could reduce significantly the blood glucose levels and improve the disorders of glucose metabolism in T2DM rats.4, Compared with the model group, TFMP70mg/kg rat serum TC, TG, LDL-c levels were decreased significantly (P<0.05 or P<0.01), HDL-c were increased significantly (P> 0.05), the results have indicated that TFMP could improve the disorders of blood lipid metabolism in T2DM rats.5, Compared with the model group, TFMP 70 mg / kg has increased activity of SOD and decreased MDA content significantly in serum (P <0.05), From the result we can conclude that PDS can increase the activity of SOD in T2DM rats, improve the ability of eliminating free radical, which was good to preventing the incidence of Diabetes Mellitus.6, compared with the normal control group, the content of IL-1β, IL-6 and TNF-αin T2DM rats has increased significantly (P <0.05 or P <0.01). TFMP 70mg / kg have decreased IL-1βand IL-6 levels compared with the model group (P <0.05, P <0.01), TNF-αhave be in a downward trend, but there was no significant difference (P> 0.05). The results suggested that TFMP could reduce the damage caused by inflammatory response in the body.7, compared with the normal control group, the C-peptide and insulin levels of T2DM rats were significantly lower, and islet cells were dispersed. The distribution of isletβcells was uneven, and the number of islet cells was decreased significantly, C-peptide and insulin levels were significantly increased after administered TFMP, and insulin secretion index (IS) was increased significantly, the damage of islet have be better improved.This study have showed that: TFMP could decrease high blood sugar caused by adrenaline, but the specific mechanism to be studied further. TFMP could treat type 2 diabetes, its mechanism may be related to that it could increase glycogen synthesis, regulate the disorders of glucose and lipid metabolism, reduce the inflammatory response, improve the ability of eliminating free radical and improve isletβ-cell function and so on. This research can make a theoretical basis on the study TFMP in the further development and application.