The Study Of Leptin Gene Polymorphisms G-2548A In Patients With Coronary Heart Disease

Objective: To explore whether single nucleotide polymorphism G/A in the promoter of leptin gene is related to CHD in Chinese Han people of Hubei province. To study the association between the different genotypes. To evaluate the association between the plasma leptin level with coronary heart disease (CHD), and to verify the correlation with the genotype distribution and the plasma leptin level.Method: (1) 156 patients with clinical diagnosis of CHD or positive findings for CHD in coronary angiography were grouped as CHD group, whereas 150 subjects with negative ECG changes for CHD or normal coronary angiography were grouped as normal control group. (2) Serum leptin levels in all subjects included in this study were measured by radioimmunoassay method. (3) The genotypes of leptin were detected by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) methods in 156 CHD patients and 150 normal controls.Results: (1) The levels of plasma leptin in the CHD patients were higher than those in the normal control. (2) There was significant difference in frequencies of allele in G-2548A polymorphism between CHD and control group (CHD : controlχ2=11.55,P<0.01). The relative risk suffering from CHD with genotype AA+AG was 3.827 times of those with genotype GG (OR=3.827; CI=1.672～8.756).(3) In CHD group, patients with AA genotype showed higher fasting leptin level than those in the AG and GG genotypeConclusion: (1) In CHD group, leptin gene promoter had different distribution of genotypes compared with the normal controls. (2) Leptin gene polymorphism G-2548A was significantly correlated with CHD.'A'allele of leptin might be a genetic factor that may contribute to individual susceptibility for CHD. (3) In CHD groups, serum leptin level in AA genotype was higher than in AG and GG genotypes.