The Effect Of Gan Yanping On Norepinephrine And Dopamine In Vivo Of Rats With Liver Fibrosis

Objective:To investigate the effect of Gan Yanping on norepinephrine(NE) and dopamine(DA) in liver tissue and plasma and the possible mechanism resisting hepatic fibrosis induced by carbon tetrachloride (CCl4).Methods:The 53 rats were randomly divided into control, model, GYP-treated and Heluoshugan-treated group. The changes of liver tissue on pathohistology and ultrastructure were observed, futhermore the NE and DA concentrations in liver tissue and plasma were determined with high performance liquid chromatography- electrochemical detector (HPLC-ECD).Results:The levels of NE in model, GYP-treated group and Heluoshugan-treated group were obviously less than in normal group(P<0.01), and NE in model group was less than in GYP-treating group(P<0.05) and Heluoshugan-treated group(P<0.05); while DA was similar in the four groups(P>0.05).The levels of NE in GYP-treated group and normal group were obviously less than in model group(P<0.01), and the level of NE in Heluoshugan-treated group was also less than in model group(P<0.05). The levels of DA in model group and Heluoshugan-treated group were obviously less than in normal group and GYP-treated group(P<0.01), while the levels of DA in Heluoshugan-treated group and model group were similar(P>0.05).Conclusions:Gan Yanping could increase the NE concentrations in liver tissue and the DA concentrations in plasma, while reduce the NE concentrations in plasma. Therefore GYP achieved to inhibit the hepatic fibrosis mainly by regulating the levels of NE and DA in rats with hepatic fibrosis.