The Establishment Of The Multi-dimensional High Performance Liquid Chromatography And Its Application In The Qingkailing Quality Control

Due to the numerous ingredients and complicated characters of Traditional Chinese Herbs, a large quantity of related information has lost by using a single separation model, since it can hardly solve the separation and detection problems, though fingerprint spectrum has, to a certain degree, elevated the quality of Chinese Herbs and its preparations. Selecting Qingkailing injection as the analyzing sample, our goal is to use multidimensional HPLC to detect and analyze Qingkailing injection fleetly and completely. Analyzing targets cover high polarities, such as, amino acid and nucleotide, middle polarities, such as, organic acid, geniposide, baicalin, low polarities, such as, bile acid.This experiment contains three parts:1. The establishment of multidimensional HPLC. Under the guidance of multidimensional HPLC theory, we assumed different linking patterns, put each of them into practice to analyze respective effecting factors, and finally institute a proper linking model for analyzing the sample of Qingkailing injection. Reviewing the column, mobile phase, detecting condition of chromatography, we established the specific chromatography for Qingkailing sample, depending on the components (6 sorts, 11 species) of Qingkailing as index (nucleotide: adenosine, guanosine; organic acid: caffeic acid, chlorogenic acid; amino acid: phenylalanine, tryptophan; secoiridoid compounds: geniposide; bile acid: hydroxycholic acid, chenodeoxycholoc acid, cholic acid; baicalin)2. Methodology inspection of multidimensional HPLC. Through the experimentation of system adaptability, precision, stability, linearity, detection limit, quantity limit, recovery ratio, we testified that this model can well accord with the request, and can be used in the multi-ingredients quantitative analysis.3. By using the technique of Multidimensional HPLC, we carried out quantitative analysis on the 11 index components of Qingkailing injection with ultraviolet detector and evaporation light detector while we put up the relevant qualitative analysis with MS detector and electrochemical detector. As a result, the quality standards of this preparation have been elevated. Meanwhile, we carried through academic discussions on the established multidimensional HPLC technique, including the optimization of transfer technique, chromatography parameter, which indicated that a solid foundation for future research work has been settled. In conclusion, this research has established the multi-dimensional HPLC technique specifically suited to Qingkailing injection. We improved the quality standard of Qingkailing injection by the application of this technique. What's more, it supplied a train of thought to the fleet and complete analysis of Traditional Chinese Herbs and its preparations. The relevant theoretical and practical discussions and researches settled a solid foundation for the research on the multi-dimensional HPLC analysis on the ingredients of Chinese medicine.