| BackgroundWith the increasing survival rate and improving quality of life after small bowel transplantation (SBT), SBT is regarded as the most effective treatment for intestinal function failure. However, small bowel is sensitive to ischemia and reperfusion (I/R) injury, which restrict the clinical application of SBT. Therefore, it is very popular to investigate the methods to prevent and treat the small bowel I/R injury.Mannitol, a clinical drug, is widely used as a diuretic. Currently, many researches indicate that mannitol has protective effects on I/R injury of different organs. It is due to its function that can scavenge free oxygen radicals. So this research is designed to investigate the protective effects of mannitol on rat small bowel I/R injury after transplantation.ObjectivesTo investigate the protective effects of mannitol on rat small bowel ischemia and reperfusion(I/R) injury after transplantation and the possible mechanism.MethodsThe models of small bowel I/R injury after transplantation were established in rat. Male Spraegu—Dawley rats were randomly divided into three experimental groups:sham operated group, I/R group and mannitol treated group. Rats were pretreated with 20% Mannitol Injection (10ml/kg,iv) through the tail vein within 60min. We use an infusion pump to control the time. In the I/R group, 20% Mannitol Injection was replaced by normal saline in the same volume. The intestinal tissue collected at each time point were observed in histopathology way. The contents of Malondialdehyde (MDA) and superoxide dismutase (SOD) in specimens of the intestine were observed at the same time.ResultsAdministration of mannitol could relieve both the intestinal tissue damage and the changes of the contents of these two biochemical indicators significantly. ConclusionsMannitol has been demonstrated to have protective effects on rat small bowel I/R injury after transplantation, and the possible mechanism might be that mannitol could scavenge free oxygen radicals.
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