Studies On Chemical Constituents Of Altheae Rosea Flower

Objective:This topic choose the white althaea rosea flower to conduct the chemical composition research.Methods:The 95% ethyl alcohol extraction concentration extracts, adds few water and forward extract uses the benzine, the chloroform, the ethyl acetate and the normal butanol. To the chloroform, the ethyl acetate, the normal butanol part uses the silica gel column, preparation classics separation methods and so on thin layer chromatograph, crystallization to carry on the separation and purification .Results :Obtain 7 compound, using the modern spectrum technology (UV, 1H-NMR, 13C-NMR) and unifies chemistry method to carry on the analysis to the compound structure, identified 6 compound, 5 compound for flavanone glucoside compound .The 6 compound respectively are: Tiliroside ,Astragalin, Rutin, sinensin, Dihy droquercetin-4′-O-β-D- glucoside,β-sitosterol-3-O-β-D-glucopyranoside. And the sinensin,Dihydroquercetin -4′-O-β-D- glucoside be in the plant reported for the first time. In addition this topic establishes the HPLC method to determine the content of tiliroside in altheae rosea flower.this method fast, keen, the sample preparation is simple, the average returns-ratio is 98.1%, RSD=1.1%.Conclusion: The studies not only supplied and perfected the research of the chemical constituents on altheae rosea flower but also established firm foundation for the further devolopment of altheae rosea flower. At the same time the experimental results provide the data of quality standards of the altheae rosea flower.