| Objective:To dectect the protein expression of TGF-β1,MMP-2 and TIMP-1 and to study the molecular pathological mechanism of renal fibrosis and its significance for treament in the Chronic allograft nephropathy(CAN) with fibrosis.Methods:21 cases kidney specimens were collected from patients with CAN and 10 normal kidney specimens of non-nephropathy patients.HE and immunohistochemical staining were performed to detect pathological stage according to Banff pathological standard for renal fibrosis and protain expression of TGF-β1,MMP-2 and TIMP-1.An image quantitative assessment was carried out by stereological to analysis expression of TGF-β1,MMP-2 and TIMP-1.At the same time serum creatinine(Cr) and urea nitrogen(BUN) were detected.. Results:In the early staging of renal fibrosis(1 stage) positive expression of TGF-β1,MMP-2,TIMP-1 were revealed stronger than normal kidney tiassues(0 stage).With renal fibrosis increasing,expression of TGF-β1 and TIMP-1 protain were revealed a increasing gradually positive staining,and there are significant difference between the fibrosis groups and normal one(p＜0.01).Positive staining of MMP-2 was gradual weak and low even negative.There are significant difference between fibrosis groups and normal one for MMP-2 expression(p＜0.01).It showed that there was positive correlation between expression of TGF-β1 and TIMP-1 and negative correlation between expression MMP-2 and TIMP-1 and no correlation between TGF-β1 and MMP-2.With renal fibrosis progressing,the level of serum creatinine(Cr) and urea nitrogen(BUN) were higher by degree.Conclusions:It suggested that TGF-β1,MMP-2 and TIMP-1 maybe participated in the genesis and development of renal fibrosis.Higher bioactivity of TGF-β1,TIMP-1 may be important factors in renal fibrosis,and function of MMP-2 was reverse to TGF-β1,TIMP-1.It is valuable o detect TGF-β1,MMP-2,TIMP-1 and the level of serum creatinine(Cr) and urea nitrogen(BUN) for judgement stage of renal fibrosis... |
