Study On Application Of Fluorescence Analysis In Antibioticsical Detection

Luminescence analysis is the abbreviation of molecular spectrum,composing of molecular fluorescence phosphorescence and chemluminescence analysis(including bioluminescence).Fluorescence is a photoluminescence phenomenon. It is one of the most sensitive analysis techniques. There are several characteristics of fluorescence, which are high selective, less sampling, simple and rapid. The paper Reviews the application of fluorescence in pharmaceutical analysis.In this paper, we determine the amount of Cephalosporins by derivative fluorescence spectroscopy with complex reaction. The thesis consists of four parts:Part 1 Determination of Pharmaceutical by Derivative Fluorescence spectroscopyChapter 1 Determination of Ceftriaxone in Injection by Fluorescence s pectroscopyCeftriaxone sodium does not have the feature of fluorescence. The new method is based on a chromogenic reaction of ceftriaxone sodium with ferric under the condition which produces a fluorescence substance. The best emission wavelength and excitation wavelength of the fluorescence substance are 378 nm and 487 nm. The linear range of the determination is 1-10 mg·L-1. The detection limit for ceftrisxone sodium is 0.23 mg·L-1. The average recovery is 100.43%. The people were satisfied with the result.Chapter 2 Fluorimetric determination of ceftazidime in injectionThe new method is based on a chromogenic reaction of ceftazidime sodium with ferric under the condition which produce a strongly fluorescence substance. The best emission wavelength and excitation wavelength of the fluorescence substance are 370 nm and 432 nm. The linear range of the determination is 2.5-25.0 mg·L-1. The detection limit for ceftazidime sodium is 0.38 mg·L-1. The average recovery is 101.4%.Part 2 Fluorimetric study on the Complex Reaction in Pharmaceutical analysisChapter 1 Study on Fluorimeritric Determination of Cefradine capsuleCefradine emit weak fluorescence in acid sodium.The new method is based on a chromogenic reaction of Cefradine sodium with Au (Ⅲ) under the condition which produce a strongly fluorescence substance. The best emission wavelength and excitation wavelength of the fluorescence substance are 353 nm and 433 nm. The linear range of the determination is 1-25.0 mg·L-1. The detection limit for Cefradine sodium is 0.30 mg·L-1. The average recovery is 101.7%.Chapter 2 Study on Fluormeritric Determination of Cefotaxime Injection The new method is based on a chromogenic reaction of cefotaxime sodium with ferric under the condition which produces a fluorescence substance. The best emission wavelength and excitation wavelength of the fluorescence substance are 370 nm and 480nm. The linear range of the determination is 1.0-24.0 mg·L-1. The detection limit for ceftrisxone sodium is 0.18 mg·L-1.The average recovery is 100.43%. The method has been used to determination in tablets and the results were in good agreement with those obtained by classical method.Part 3 Inhibitory Kinetic Fluorimetric Determination of Cefradine An inhibitory kinetic fluorimetric method is reported for the determination of Cefradine. The proposed method is based on the inhibitory effect of Cefradine on the reaction of (NH4)2Ce(NO3)6 oxidation of Lucigenin in Na2SO4-H2SO4 buffer solution medium. The detection limit for Cefradine is 1.09 mg·L-1.The linear range of the determination is 2.5~50 mg·L-1. This method has been used successfully to determine Cefradine capsules.Part 4 Study on the Determination of Amikin with Rayleigh Scattering SpecterumIn weak acid medium, Amikin or ChromeAzcerol S alone can only produce very weak resonance Rayleigh scattering (RRS) signal. However, when AMK and CAS react with each other to form the ion association complexes, the RRS intensity can be enhanced greatly, which gives a new RRS spectrum The maximum scattering peak is at 414 nm and there is a linear relationship between the RRS intensity and the AMK concentration in the range of 1.2～10.0 mg·L-1. The detection limit for amikin is 0.19 mg·L-1.The average recovery is100.2%.