Study On The New Determination Method For Antiviral And Cardiovascular Drugs By Fluorescence And Resonance Scattering Technique

Resonance Rayleigh scattering(RRS) and resonance non-linear scattering(RNLS) were a new technology developed in 1990s.Due to its remarkable characteristics of high sensitivity and simple operation,this method has received much attention.In this thesis,taking antiviral and cardiovascular drugs as research objects,we developed some new systems and new methods for the determination of them by RRS and RNLS methods.The spectral characteristics were discussed combining with the absorption and fluorescence spectra.The optimum conditions and the influencing factors were investigated.The reaction mechanisms were discussed and some new analytical methods were established.Main investigated systems are listed as follows:1.Resonance Rayleigh Scattering Study of the Interaction of Palladium(â…¡)-Moroxydine Hydrochloride chelate with Eosin Y and Their Analytical ApplicationsIn pH 4.4～5.0 acidic medium,Palladium(â…¡) reacted with moroxydine hydrochloride to form the chelating cation[Pd(ABOB)]²⁺,which further reacted with eosin Y(EY),erythrosine(Ery) and dibromofluorescein(DBF) to form ion-association complexes.As a result,the new resonance Rayleigh scattering(RRS) spectra appeared and scattering intensities enhanced greatly.The maximum RRS peaks of three systems were located at 315 nm.RRS intensity was proportional to the concentration of moroxydine hydrochloride(ABOB) in the rang of 0.012～1.2μg·mL^-1(EY system), 0.23～2.3μg·mL^-1(Ery system) and0.24～1.5μg·mL^-1(DBF system),and the detection limits(3s) were 0.0036μg·mL^-1(EY),0.070μg·mL^-1(Ery) and0.025μg·mL^-1(DBF). The optimum conditions of the reaction and the properties of analytical chemistry were investigated.Therefore,a new method for the determination of moroxydine ion-association complexes.As a result,the fluorescence intensities of dyes were quenched greatly.The maximum excitation and emission wavelengths(λ_ex/λ_em) of three systems were at 518 nm/542 nm(EY system),λ_ex/λ_em=276 nm/305 nm(Ery system) and 506 nm/533 nm(DBF system).The fluorescence quenching intensities were proportional to the concentration of ABOB in the rang of 1.5～2.3μg·mL^-1(EY system),2.5～5.0μg·mL^-1(Ery system) and 0.05～4.0μg·mL^-1(DBF system).The detection limits(3s) of three systems were 0.021μg·mL^-1,0.21μg·mL^-1,0.071μg·mL^-1.The optimum conditions of the reaction and the effects of coexisting substance were investigated.The method was applied to the determination of moroxydine hydrochloride(ABOB) with satisfactory results.4.Fe³⁺-Aciclovir Tablets-K₃[Fe(CN)₆ SystemIn dilute HCl solution medium,Fe³⁺ could be reduced to Fe²⁺ by aciclovir and Fe²⁺ could further react with[Fe(CN)₆]^3- to form Fe₃[Fe(CN)₆]₂,which led to the significant enhancement of RRS spectrum.RRS intensity was proportional to the concentration of aciclovir in the rang of 0.05～4.0μg·mL^-1,the detection limit(3s) was 0.038μg·mL^-1. The method had high sensitivity and good selectivity,which was applied to the determination of aciclovir in uric samples with satisfactory results.5.Resonance Rayleigh Scattering Spectra of Propafenone Hydrochloride-Erythrosine Systems and Their Analytical ApplicationsIn pH 4.4～5.0 HAc-NaAc buffer medium,Propafenone Hydrochloride(PPF) could react with Ery and DBF to form ion-association which led to the appearance of new RRS spectrum and significant enhancement of RRS intensity.The spectral characteristics of the reaction products were similar,and the maximum wavelengths were all at 300nm.The detection limits(3s) were 0.021μg·mL^-1(Ery system),0.075μg·mL^-1(DBF system).RRS intensity was proportional to the concentration of aciclovir in the rang of 0.5～2.5μg·mL^-1(Ery system) and 0.5～2.5μg·mL^-1(DBF system).The method had high sensitivity and good selectivity and was applied to the determination of propafenone hydrochloride samples with satisfactory results.6.The Fluorescence Spectra Study of Propafenone Hydrochloride-Erythrosine Systems and Their Analytical ApplicationsIn pH 4.4～5.0 HAc-NaAc buffer medium,Propafenone Hydrochloride(PPF) could react with Ery and DBF to form ion-associations which resulted in the fluorescence quenching of Ery and DBE The fluorescence quenching intensity was proportional to the concentration of PPF in the rang of 0.5～3.5μg·mL^-1 and 0.5～3.0μg·mL^-1 for Ery and DBF systems,and the detection limits(3s) were 0.045μg·mL^-1 and 0.087μg·mL^-1,respectively.The optimum reaction conditions and the effects of the coexisting substance were investigated.The method was applied to the determination of propafenone hydrochloride(PPF) with satisfactory results.]...