Effect Of Bunge Pricklyash Seed Oil On Airway Remodeling In Rat

Aim and significanceAsthmatic airway remodeling is the most important pathologic charater of brochial asthma. The process of airway remodeling included airway smoothm muscle hyperplasia, basement membrane thickness, gland hyperplasia, subepithelial fiber hyperplasia and collagen deposition and so on, which could cause airway wall thickness and airway lumina stenosis.Airway remodeling could cause inreversible airflow blockage and persistent airway hyperresponsiveness, which may be the pathologic basis of repeated attack of refractoriness asthma, and study them could be in favour of early stage of asthma prevention and curation, and aggravation of asthma. Nowadays we evaluate the effect of any drug preventing and treating asthma not only by its effcacy of preventing wheeze,but also by the effect in preventing airway remodeling.Oil from bunge pricklyash seed has a long histry of treating asthma with good therapeutic effect. Nowadays many national institutions successively start to study the effect of bunge pricklyash seed oil treating asthma, and had got a lot of achivements[1-2]. A lot national reports and ourselves' study outcomes confirmed the effect of bunge pricklyash seed oil in treating asthma not only in molecular biological aspect but also cilinic aspect.But so far no study had reported bunge pricklyash seed oil could prevend the airway remodeling. We choose some factors associating with airway remodeling to build rat models of asthma, and try to reveal the underlined mechanism of bunge pricklyash seed oil preventing airway remodeling in asthma,in order to supply the theoretial basis of better usage of bunge pricklyash seed oil in preventing asthma.Content1. Study the effect of bunge pricklyash seed oil to III and V type collagen in airway-remodel rat models of asthma.2. Study the effect of bunge pricklyash seed oil to PCNA in airway smooth muscle in airway-remodel rat models of asthma.3. Study the effect of bunge pricklyash seed oil to the expression of TGF-β1 in airway-remodel rat models of asthma.Material and methodsHealthy SD rat(n=30),200-250g.All animals were randomly divided into 3 groups:asthma group(A group, 10),treat group(B group, 10)and control group(C group, 10). All animals were subcutaneous injected in forelegs with 0.5ml 1% OVA 0.5ml 1% AL(0H)3, and repeat it again on day 8.On day15 all animals inhaled with 1% OVA for 5min.We prepared OVA ereryday and put rats in a container to aerosol rebreath. The rats from treatment group were gave 3ml/kg mixture of bunge pricklyash seed oil and essential oil(1:1) by intragastric administration one hour before aerosol rebreath. Rats of control group were gave NS instead of OVA. All animals treated to death 3 monthes after provocation. Results1.Lung tissue HE stain: Section preparations of asthmatic rat showed that epithelium of bronchi were engorged and ablated, and the wall of airway was thickened and calibre was stenosis. Airway wall and surronding tissues were infaltrated with inflammatory cells, of which EOS and lymphocyte take the advantage.Lung tissue of rats of treatment group showed that less severe inflammation in bronchial epithelium,and the inflammatory cells in airway wall were also reduced.Rat of control group had no such apparance .2.Changes in thickness of rat airway smooth muscle :the results of image analysis showed:section preparations was amplified 100 times.Conclusion1.Bunge pricklyash seed oil could decrease the extent of inflammation and thickness of airway wall in airway-remodeled rats.2.Bunge pricklyash seed oil could decrease the expression of III and V type collagen in airway-remodeled rats.3.Bunge pricklyash seed oil could decrease the expression of TGF-β1 in airway-remodeled rats.4.Bunge pricklyash seed oil could decrease the expression of PCNA in airway-remodeled rats.All of the four points above indicated that Bunge pricklyash seed oil could not only treat asthma effectively, but also prevent airway remodeling effectively.