Value Of ThinPrep Bronchial Brushing Cytology In The Type Diagnosis Of Lung Cancer

Objective: To evaluate ThinPrep bronchial brushingcytology in the subclassification of lung cancer. Methods: Selected1365 samples of ThinPrep bronchial brushing cytology and collectedrelated clinical materials or/and pathological diagnosis. Results:There were 969 samples with clinical or/and pathological diagnoses. Thesensitivity and specificity of ThinPrep bronchial brushing cytologywere 82.7ï¼…and 96.2ï¼…respectively. The accordant rates of cytology insubclassification were 94.8ï¼…in squamous cell carcinoma, 92.3ï¼…inadenocarcinoma and 97.4ï¼…in small cell lung carcinoma. Respectively, there were 95.7ï¼…and 59.3ï¼…of poorly differentiated carcinomas inwrongly and non subclassified cytology. Conclusions: ThinPrepbronchial brushing cytology had high sensitivity and specificity indiagnosis of lung cancer and high accordant rate in subclassifica-tion,but type diagnosis of poorly differentiated carcinomas can bedifficult sometimes when based on the ThinPrep bronchial brushingcytology alone. Objective: Helping subclassifying lung cancers in ThinPrepbronchial brushing cytology by immunocytochemistry.Materials and Methods: We collected remained samples of 206positive bronchial brushings cytology and 45 fine needle aspirationsamples of resected lung tumors and examined antibodies of CK10&13,CK7,CK18,CD56 and SYN in lung cancers by immunocytochemistry.Result: Giving up 59 samples which have less than 10 tumor cellsper region of each smear, remained 151 bronchial brushings samples and41 fine needle aspiration samples. Positive rates of CK10&13,CK7,CK18,CD56 and SYN were 94.7ï¼…,38.6ï¼…,67.3ï¼…,10.5ï¼…and 7.1ï¼…in squamouscell carcinoma, 29.7ï¼…,98.6ï¼…,98.6ï¼…,22.7ï¼…and 5.9ï¼…inadenocarcinoma, 12.5ï¼…,37.5ï¼…,83.3ï¼…,86.3ï¼…and 81.6ï¼…in small cell lungcarcinoma respectively. The sensitivity and specificity of theantibodies were 94.7ï¼…and 72.0ï¼…of CK10&13 in squamous cell carcinoma,98.6ï¼…and 61.5ï¼…of CK7 and 98.6ï¼…and 31.0ï¼…of CK18 in adenocarcinomarespectively, 86.3ï¼…and 82.9ï¼…of CD56 and 81.6ï¼…and 93.5ï¼…of SYN in smallcell lung carcinoma respectively. Additionally there were no significantdivergences of these antibodies in different differentiated tumors.Combining cytology and ICC in diagnosis of unclassified lung cancers, the sensitivity of squamous cell carcinoma,adenocarcinoma and smallcell lung carcinoma were 90.0ï¼…,96.3ï¼…and 100ï¼…respectively. In theinvestigation, we found that besides CK7 and CK18, columnar cells werealso positive for CK10&13,CD56 and SYN in different degrees.Conclusions: Combining cytology and ICC can improve the accuracyof subtype of lung cancer and ICC may become a supplementary method forsubclassification of lung cancer.