| Background and objective Peritoneal meatastasis is the most frequent cause of death in patient with gastric cancer. Detection of free cancer cells in the peritoneal cavity at the time of surgery, therefore, is considered to be of great value in predicting the peritoneal recurrence and accordingly in the progrosis of patients with gastric cancer. This study investigate the clinical significance of intraoperative determination of CEA\B72-3 expression and routine cytologic examination of free cancers in peritoneal lavage fluid and gastric wall brushing smears in gastric cancer .In order to find a more rapid convient intraoperative method for predicting peritoneal metastasis.Method The peritoneal lavage fluid and gastric wall brushing smears were sampled during laparotomy from 44 patients with gastric cancer and 10 patients with benign gastric disease .CEA B72-3 were detected by immunocytochemistry(S-P)and cytology detected by the conventional method respectively, meanwhile, several realated clinicopathologic factors was retrospectively analysed.Result The positive rate of cytology CEA B72-3 expression was 25.0%(11), 36.3%(16), 38%(17)respectively in gastric wall brushing smears. While cytology,CEA,B72-3 were positive in 8(20.8%),14 (31.8%), 14(31.8%) cases of peritoneal lavage respectively. The difference had no statistical significance but the correlation was existed between two groups. B72-3 CEA immunocyto-chemistric analysis especially when used in combination, was more sensitive than cytologic examination, with an average 14% improvement. Lymph node metastasis, depth of invasion, serosal involvement and TNM stage were correlated with the positive rate of cytology, CEA, B72-3.Conclusion The combined use of CEA, B72-3 immunocytochemistric analysis in gastric wall brushing smears can serve to predict patients who will develop peritoneal metastasis... |
PDF Full Text Request