Influence Of Macrophage Co-culture To Fertility Rate And Blastocyst Formation Rate Of The Mouse's Oocytes

The Influence of macrophage co-culture to the fertility rate and blastocyst formation rate of the mouse's oocytes.Objective:1. To setup a mouse IVF model.2. To investigate the effect of co-culture with peritoneal macrophage to the fertility rate and blastocyst formation rate of the mouse's oocytes.Methods:1. HMG 5IU, 10IU and 15IU were used in KunMing mouse aged 7-8 weeks, 10-14 weeks and over 15 weeks, weighted 32 gm, 32-40gm and over 40gm, respectively. HCG 10 IU was given 48 hours after HMG. Egg collection was performed 16-20hours later to evaluate the quantity and quality of the egg.2. A total of 15 KunMing mouse aged 10-14 weeks were undergone ovulation induction and the oocytes were collected and randomly divided into three groups. The first group was the control group and cultured alone with no macrophage; the second group(experimental group 1) were co-cultured with macrophages right after the egg collection; the third group(experimental group 2) was cultured alone after egg retrieval, and co-cultured with macrophages from 8 cell stage.Results:1. The biggest amount and the best quality of egg were found in Kunming mouse of 10-14 weeks of age and 32-40 gm of weight with HMG 10IU and HCG 10IU.2. The fertility rate in experiment 1(72.0％) was significantly lower than that in control group(91.0％and 93.7％) in first group and experiment 2 respectively, (p＜0.001).3. Blastocyst formation rate of the fertilized oocytes in experimental group 2 (78.2％) was much higher than control group (50.3％, p＜0.001).4. Blastocyst formation rate of the oocytes in experimental group 2(73.2％) was much higher than control group(54.8％) and experimental group 1(50.0％); While the rate in experimental group 2 was the highest among the 3 groups, which was statistically significant.Conclusion:1. Co-culture with peritoneal macrophages had an adverse influence on the fertilization process.2. Peritoneal macrophages could enhance the mouse embryonic development and increase the blastocyst formation rate.