| Objective: To study methods raising average percentage of ferti- lization by intracytoplasmic sperm injection (ICSI) in mouse eggs. To study oocyte activation capability of different parameters human semen samples.Methods: KunMing white mice (8~10 weeks of age) were used as donors of eggs. The influence of different phases of collecting eggs and different culture time on ICSI was investigated. Semen samples according to WHO (1994) recommendation were evaluated, and were treated by SWIM-UP.Results: 1. The mature rate of mouse oocyte, which the collection of oocytes is done 18 hours after the injection of HCG, is significant higher than the other groups (P<0.05).2. The oocyte activation rate and normal morphy rate in the group which culture time is 2 hours is higher than 4 hours. The difference is significant (P<0.05). 3. The activation rate of the experimental controlled group and the opening controlled group are significant lower than the experiment group (P<0.01).4. The survival rate of the opening controlled group is higher than the other groups (P<0.05).5. No significant difference on activation rate occurred between different groups of sperm (P>0.05).Conclusion:1. The proper time of the oocyte collecting and the oocyte culture is important for the result of ICSI.2. It might be suggested that the mouse oocyte activation capacity of human sperm do not directly relate to initial semen sample results.3. The fertilization rate of the mouse oocyte after ICSI is low. The volume and the ability of resistence of injury to mouse oocyte may result in the above results.4.The failure of oocyte... |
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