Studies Of The ATP Content Of Unfertilized Oocyte, Undivided Zygote And Embryo Unsuitable To Transfer And Cryopreservation

OBJECTION To analyse the difference of ATP content of unfertilized oocyte,undivided zygote and embryo unsuitable to transfer and cryopreservation, and to explore the relationship between ATP content and developmental potential of oocyte.METHODS1.72 unfertilized oocytes,26 undivided zygotes and 70 embryos unsuitable to transfer and cryopreservation were recruited from IVF-ET cycles during a period from August 2009 to January 2010.2. (1) All samples were divided 3 groups:unfertilized oocyte group, undivided zygote group and unsuitable to transfer and cryopreservation embryo group.(2) oocytes and embryos were divided 3 groups respectively according to age:≤29 years group,30～34 years group and≥35 years group.(3) oocytes and embryos were divided 2 groups respectively according to the out-come of IVF:pregnant group and non-pregnant group.(4) embryos unsuitable to transfer and cryopreservation were divided 2 groups:Ⅲ,Ⅳembryo group and polypronuclear embry group. 3. The ATP-bioluminescence assay (ATP-CVA) was performed to detect the ATP content.Compare the difference of ATP content between groups to verify whether there is any statistic significance.RESULTS1. ATP content in unfertilized oocyte, undivided zygote and embryo unsuitable to transfer and cryopreservation were 1.82±0.191 pmol,1.98±0.240 pmol,3.00±0.297 pmol, respectively. There was significant difference between groups, P<0.01.2. There were no significant differences in ATP content of oocyte and embryo between≤29 years group,30～34 years group and≥35 years group,P>0.05.3. ATP content of oocyte and embryo in the pregnant group were significantly higher than the non-pregnant group(oocyte:1.90±0.189 pmol vs 1.75±0.167pmol, P<0.01; embryo:3.12±0.319pmol vs 2.89±0.234pmol,P<0.01).4. ATP content inⅢ,Ⅳembryo group were significantly lower than polypronuclear embryo group(2.88±0.276pmol vs 3.17±0.237pmol,P<0.01).CONCLUSION1. Reduced ATP content may be related to failures in oocyte fertilization, zygote division and abnormal embryo development.2. There was significant difference in ATP content between pregnant group and non-pregnant group, which indicant that mitochondrial function was correlated with individual fertility.