Studies On Quality Evaluation Of Xinqin Kili Granule Using ～1H-NMR Spectroscopy Combined With Pattern Recognition And The Chemical Constituents From The Roots Of Ardisia Brevicaulis Diels.

Chinese medicine as the material base of preventing and treating diseases made a tremendous contribution for the prosperity of the Chinese nation reproduction. But due to lacking of the stable and controllable quality standards, Chinese medicine had not been allowed to enter the international pharmaceutical drugs market. Thus the goverment prefered the strategy named "Modernization of Traditional Chinese Medicine", and formulated a series of quality control projects. The main methods included the use of microscopic identification for herbs organizational structure, TLC(thin layer chromatography) identification, HPLC(high performance liquid chromato -graphy) identification and the fingerprint based on quantitative determination with HPLC. These methods rely on the results of identifying and determining contents of the reference components to evaluation the quality of Chinese medicine, which played a pivotal role. However, these methods couldn't study the overall composition. Therefore to establish a method which could reflect the inherent quality of the traditional Chinese medicine has a very important significance.Therefore, NMR (nuclear magnetic resonance) fingerprint emerged as a new method to analyze the all composition of Chinese medicine. It is a new comprehensive monitoring of the quality of medicines using NMR combined with pattern recognition technology.The first part of the dissertation deals with the quality evaluation of Xinqin Kili granule and its main composition (Xixin, Huangqin and Huangqi) using ~1H-NMR spectroscopy combined with the similar analysis and principal components analysis methods. Its purpose is to establish a reasonable and feasible quality control method of traditional Chinese medicine.The second part of the dissertation deals with the chemical constituents from the roots of Ardisia brevicaulis Diels.. The materials came from the roots or entire plants of Ardisia brevicaulis Diels., and mainly located in Hubei, Sichuan, Guizhou, Jiangxi, Guangxi, and other places. It is mainly used to expelling wind and removing heat, eliminating stasis to subdue swelling. The literature on the chemical constituents from this plant has not been reported. 19 compounds were isolated by repeating silica gel and Sephadex LH-20 column chromatography, in which, 18 were identified. Three new compounds were identified as 5-tridecyl-6-O-acetate-3- hydroxyl-1-methyl phenyl ether; 4-tridecyl-3,5-O-acetate-1-methyl phenyl ether; 2-pentadecyl-6-methoxy-3-[2'-methyl-5'-(14l,15'-pentadecenyl)-4',6'-resorcinol] -1, 4-benzoquinone. All the compounds were obtained from this plant for the first time.The third part of the dissertation is a review to introduce the pattern recognition technology in the identification and quality control applications of traditional Chinese medicinal materials. This article summarized the progress, the main research methods, and the application of the pattern recognition technology in the identification and quality control of Chinese herbal medicine in recent years.