Present Condition Of P.Acnes Drug Resistance To Antibiotics

Objective Propinonibacterium acnes (p.acnes) is an important pathogenic bacterium, and its new popular trend and new characteristic of drug fast shows up continuously under the present pressure of new broad-spectrum antibiotics. We try to know the present condition and the changeable state of drug resistance of P.acnes to antibiotics through examining the minimal inhibitory concentration of P.acnes to erythromycin,arilin, clindamycin and tetracycline in order to provide the best antibacterials for clinic medication therapeutics.Methods This study was mainly from the following aspects of exploration:First, we collected 60 cases of acne patients with acne content as a specimen which were isolated and cultured, the strains will be done with colony morphology, appearance in microscopic,catalase test, nitrate test , indole test identified, VITEK automatic re-microbial analysis system, anaerobes ANI identification card identification.Secondly, chi-square test was used to compare the effect of sex and age on the p.acnes positive rate. Thirdly, agar dilution method was used to test the sensitive level of P.acnes to erythromycin, clindamycin, tetracycline and metronidazole.Results The 33 P.acnes were isolated and cultured from the 60 samples, which were passed through under colony morphology, appearance in microscopic,catalase test, nitrate test ,indole test ,VITEK automatic pilot and microbial analysis system, ANI anaerobes identification card identification, They were clearly identified. The results of the VITEK automated microbial analysis system and ANI anaerobes identification card identification are corresponding with the results of colony morphology, appearance in microscopic,catalase test, nitrate test and indole test. In this experiment, the cultural rate is 55%. The sex and the age have no effect on the cultural rate. We examine this 33 strains'sensitive level to the 4 amtibiotics by agar dilution method. The result shows that 19 strains have drug resistance to erythromycin, and 13 strains to arilin, 5 strains to tetracycline, 3 strains to clindamycin. And the drug resistance rate respectively is 57.6%, 39.4%,15.2% and 9.1%.Conclusion Drug resistance of P.acnes is exist in Chongqing area, especially erythromycin resistance rate is higher. P.acnes resistance is correlated with antibiotic unreasonable application. We should acquire pathogens prevalent trends and characteristics of resistance in clinical Dermatology. which has great significance to control the spread of resistant strains and to guide clinical use of antibiotics...