Correlation Study On The NF-κB Polymorphisms In Chinese Patients With Type 2 Diabetes

Objective Nuclear factor kappaB(NF-κB) is an important transcription factor that is involved in the response to oxidative stress and infection ,It takes part in cell growth,differentiation,adhere,apoptosis and inflammatory reaction . Recent studies suggest that it is involved in the development of diabetic great vascular complications . A highiy polymorphic (CA) dinucleotide repeat microsatellite has been identified in the regulatory region of the NF-κB gene . The aim of this study was to explore the relationship of the ( CA ) dinucleotide repeats microsatellite of the NF-κB gene with type 2 diabetes mellitus ( DM ) and diabetes coronary heart disease. Method By using PCR - fluorescence we examined CA dinucleotide repeat microsatellite on the NF-κB gene in population of 161 T2DM ( 87 patients had no CHD and 74 patients with CHD ) and 99 healthy persons as control ( CON ) group .Results 1 . A3 ( 122bp ) and A9 ( 134bp ) alleles are more common in the T2D M and CON . 2 , There was a highly significant increased in the frequency of allele A4 ( 124bp ) in CON ( 20 . 20 % ) compared with the T2DM (2.48%) (X2 =45.935 , P < 0 . 0001 ) . 3 . There was a highly significant increased in the frequency of allele A8 in T2DM (17 . 08 % ) compared with the CON ( 6. 57 % ) (X2 =11.926 , P < 0 . 0001 ). 4 . But there was no difference in the frequency of alleles between diabetic patients had no CHD and with CHD .Conclusions The NF-κB gene may play a role in the susceptibility to type 2 diabetes: individuals with the A8 allele may be more likely to develop type 2 diabetes compared with the A4 allele .