Correlation Study On The Nuclear Factor Kappa B Polymorphisms In Chinese Patients With Type 2 Diabetes

Objective Nuclear factor kappa B (NF-κB) is an important transcription factor that is involved in the response to oxidative stress and inflammation. Recent studies suggest that it may be involved in the development of diabetic microvascular complications. A highly polymorphic(CA) dinucleotide repeat microsatellite has been identified in the regulatory region of the NF-kB gene. The aim of this study was to explore the relationship of the (CA) dinucleotide repeats microsatellite of the NF-kB gene with type 2 diabetes mellitus (DM) and diabetes nephropathy (DN).Method By using PCR-fluorescence we examined CA dinucleotide repeat microsatellite on the NF-kB gene in population of 186 T2DM (101 patients had no DN and 85 patients with DN) and 82 healthy persons as control (CON) group.Results 1. A3(122bp) and A8 (132bp) alleles are more common in the T2DM and CON. 2. There was a highly significant increased in the frequency of allele A4(124bp) in CON (28.66%) compared with the T2DM (1.88%) (X~2=79.77, P<0.0001) . 3. There was a highly significant increased in the frequency of allele A8 in T2DM(31.56%) compared with the CON (11.59%) (X~2=23.21, P<0.0001) .4. But there was no difference in the frequency of alleles between patients had no DN and with DN.Conclusions the NF-kB gene may play a role in the susceptibility to type 2 diabetes and diabetes nephropathy: individuals with the A2 allele may be more likely to develop diabetes compared with the A14 allele.