Study Of Raman Spectroscopy Of Optically Trapped Single Human Red Blood Cell

Laser tweezers Raman spectroscopy(LTRS)system is a combination of Raman spectroscopy and laser tweezers;it is a new method to study cells;it can trap single living cell and make Raman spectrum of single living cell.From the positions,intensities,and line widths of the Raman peaks in the spectra,we can get useful information about composition,structure and interactions of complexes inside the living cells.External agents may change cell's physiological state and this changed information can also be got from Raman spectra.Two kinds of human red blood cell(RBC)were studied in the article.Firstly,the study of RBC cryopreserved.People can get a effective method for conserving human RBCs for a long time by cryopreserving.In the past several decades,the method of high glycerol is applied widely.It is valuable to find whether the RBCs change and what kind of change it will happen.In the article thawy RBCs were studied by the means of Raman spectroscopy using LTRS system.The changes of the Raman spectroscopy of the red blood cells cryopreserved at -80℃have been found and also have found some changes of the red blood cells in the culture liquid after they are thawed.All these are valuable for the study to cryopreserved RBCs more deeply.Secondly,This article is also a study of Raman spectra of RBCs affected by different electricity;from the result,distinct change of Raman spectra of RBC affected by direct current(DC)have been got.These changes characterize the changes of the internal information of the cells.This article give some academic reference of physical therapy using electricity in the level of molecule.