The Protection Of Lotus-seed Milk Fermented Production On The Intestinal Mucosal Barrier In Mouse

The lotus-seed milk fermented production (LMFP) can be defined as a mixture of Lactobacillus acidophilus and lotus-seed polysaccharide that is being used increasingly to denote a synergistic relationship between probiotics and prebiotics, and has the functions that are associated with a balanced colonic microflora, that modify gastrointestinal immune activity. The studies were conducted to explore the effect of the lotus-seed milk fermented production on the intestinal mucosal barrier in mouse, and to gather the information for utilizing the lotus-milk fermented production as functional foods.1,The lotus-seed milk fermented production improve the gastrointestinal functionsIn this study, 60 male ICR mice were randomly assigned into five groups, each of twelve. The mice were pretreated intragastrically with 25%, 50%, 75% lotus-seed milk fermented production and distilled water once daily for five day, then fed intragastrically with Compound Diphenoxylate to make the model of constipation, measured the time, weight, quantity and the water content of black feces excreted during 6 hours.As compared with control group, weight and water content of black feces of model group was significantly reduced (p=0.002, p<0.001), time of black feces excreted was significantly extended (p=0.001); Time of black feces excreted of 25%, 50%, 75%LMFP groups was significantly reduced than that in model group (p=0.002, p<0.001,p=0.010); Weights of black feces excreted of 25% and 50% LMFP groups were significantly increased than that in model group (p=0.008,p=0.005), water content of black feces of the all dosage LMFP groups was significantly increased than that in model group , all Significance<0.001.The results indicate the ease-constipation of the LMFP.2,The protection of lotus-seed milk fermented production on the intestinal mucosal barrier in mouseIn this study, 60 male ICR mice were randomly assigned into five groups, each of twelve. The mice were pretreated intragastrically with distilled water, lotus-seed milk fermented production, lotus-seed milk and fermented milk once daily for two weeks. Twenty-four hours after the last dosing, animals were administered with LPS(6mg/kgBW) intraperitoneally, control animals were given distilled water only. Eighteen hours after the intoxication, the targets were measured.The mice injected with LPS showed the symptoms of sluggish in varying extent, especially in model group. The LMFP group were the most slight compared with other groups. As compared with control group, The concentration of intestinal SIgA, quantity of white blood cell and Lactobacillus acidophilus of model group were significantly reduced (all p<0.001), The concentration of TNF-αand the quantity of Escherichia coli were significantly extended (all p<0.001). The lotus-seed milk fermented production can significantly improve The concentration of intestinal SIgA and the quantity of white blood cell(p<0.001,p=0.001), retain the amount of Lactobacillus acidophilus(p<0.001), decrease the concentration of TNF-αin serum induced by LPS and the quantity of Escherichia coli in the mouse's caecum. The protective effect of lotus-seed milk fermented production on the intestinal mucosal barrier harmed by LPS was significantly better than fermented milk, and there was short of protection in lotus-seed milk group.In our study, the model that intestinal mucosal barrier harmed by LPS was established successfully. The lotus-seed milk fermented production can protect the intestinal mucosal barrier against harm caused by LPS, and relieve the response of systemic inflammatory.