Effect Of Omega-3 Fish Oil On Intestinal Mucosal Barrier In SAP Rats

Objective:To study the effect of omega-3 fish oil in the intestinal mucosal barrier injury of serious acute pancreatitis, and to research its clinical application value.Methods:48 male wistar rats were randomly divided into 3 groups:sham operated group(So, n=16), fish oil supplementation treatment group(F, n=16) and normal sodium treatment group (N, n=16). To evaluate the intestinal mucosal barrier injury of serious acute pancreatitis by detecting the activity of Plasma diamines oxidase (DAO) and milk fructose/mannitol ratio in urine.Results:The content of Plasma diamines oxidase (DAO) in the group F was significantly lower than that in the group N and in the group So (P<0.01); it had also a statistical significance that a comparison of the content of DAO in the group N to that in the group So (P<0.05).The milk fructose/mannitol ratio (L/M) in urine in the group F was significantly less than that in the group N and in the group So, and it had also a statistical significance that a comparison of the ratio (L/M) in the group N to that in the group So (P<0.05).Changes in pancreatic tissues:The pancreas tissue in the group So was normal, no bleeding and necrosis by the naked eye and in the light microscope. The pathological changes in the group N were pancreatic interstitial edema, vascular congestion, inflammatory cell infiltration, the distribution of the red blood cells in tissue spaces, and disappearance of pancreas acinar structures. The pathological changes in the group F showed a large necrotic area without structure in the central area, some profiles of the pancreas lobes at the margin, secretions in the remnant pancreas acinar tissue cavities, and inflammatory cell infiltration was significantly less than that in the group N.Changes in small intestine:In the light microscope the pathological changes of small intestine in fish oil group were less than those in the group N. And the pathological score of the group F was 2.13±0.26, which had significant improvement compared with the group N, P<0.05.Conclusion:Omega-3 fish-oil fat emulsion can reduce the activity of Plasma diamines oxidase (DAO) and milk fructose/mannitol ratio in urine to protect the rat bowel mucosa barrier,thereby reducing the permeability of bowel mucosa and systemic inflammation.