| Objective To study a dynamic changes of serum von Willebrand factor and high- sensitivity C-reactive protein in stroke-prone Renovascular Hypertensive Rats. To explore the relationship between circulating levels of high-sensitivity C-reactive protein and essential hypertension initially ,and to study the effect of vWF and Hs-CRP in the deployment of atherosclerosis and the predications to stroke further.Methods 62 male Sprague-Dawley rats were randomly divided into two groups: the hypertensive group and the sham-opetated group. 50 rats were selected to make the 2-kidney, 2 clip (2k2c) renovascular hypertensive model. SBP was measured by an indirect tail-cuff sphygmomanometer (HX-1), in preheated (37°C, 15 minutes) conscious rats before renal artery constriction and every four weeks intervals after renal artery constriction for 12 weeks. The mean systolic BP in the hypertensive group were campared with sham -opetated group and among different stages . vWF and Hs-CRP were measured with enzyme linked immunoabsorbent assay( ELISA),the results were campared with the sham -opetated group and among different stages . At the end of 12 weeks after renal artery constriction, all surviving rats were killed,then observed hematoxylin-eosin stained brain sections by routine light microscopic.Results The mean systolic BP in different stages The mean SBP were 160±5 mm Hg,205±7mmHg before renal artery constriction in the hypertensive group and sham-opetated group respectively;but there were no significant difference between the two groups ( P > 0. 05); The mean SBP rose to 160±8 mm Hg,176±8 mm Hg,205±16 mm Hg at the end of 4 weeks, 8 weeks and 12 weeks after renal artery constriction in hypertensive group;The mean SBP at the end of 4 weeks, 8 weeks and 12 weeks after renal artery constriction in the hypertensive group was significantly higher than that the sham-opetated group(P<0.001); and the mean SBP at the end of 4 weeks was significantly higher than that before renal artery constriction in the hypertensive group(P<0.001) ; and the mean SBP at the end of 8 weeks was significantly higher than that at the end of 4 weeks in the hypertensive group(P<0.001); the mean SBP at 12 weeks was significantly higher than that at the end of 8 weeks in the hypertensive group (P<0.001); Plasma VWF levels in different stages The plasma vWF levels were 175.78±5.41%,176.78±4.40%before renal artery constriction in the hypertensive group and sham-opetated group respectively;but there were no significant difference between the two groups ( P > 0. 05); The plasma vWF levels were 180.45±1.65%,184.63±2.71%,187.66±1.99%at the end of 4 weeks, 8 weeks and 12 weeks after renal artery constriction in hypertensive group; The plasma vWF levels at the end of 4 weeks, 8 weeks and 12 weeks after renal artery constriction in the hypertensive group was significantly higher than that in the sham-opetated group (P<0.001, P<0.01,P<0.001, respectively); and the plasma vWF levels at the end of 4 weeks was significantly higher than that before renal artery constriction in the hypertensive group(P<0.001) ; and the plasma vWF levels at the end of 8 weeks was significantly higher than that at the end of 4 weeks in the hypertensive group (P<0.001); the plasma vWF levels at 12 the end of weeks was significantly higher than that at the end of 8 weeks in the hypertensive group(P<0.001); Plasma Hs-CRP levels in different stages The plasma Hs-CRP levels were 28.01±1.51 ng/ml,28.03±1.05 ng/ml before renal artery constriction in the hypertensive group and sham-opetated group respectively;but there were no significant difference between the two groups ( P > 0. 05); The plasma Hs-CRP levels were 29.95±1.04ng/ml,34.27±1.97ng/ml,42.58±2.35ng/ml at the end of 4 weeks, 8 weeks and 12 weeks after renal artery constriction in hypertensive group; The plasma Hs-CRP levels at the end of 4 weeks, 8 weeks and 12 weeks after renal artery constriction in the hypertensive group was significantly higher than that in the sham-opetated group (P<0.001); and the plasma Hs-CRP levels at the end of 4 weeks was significantly higher than that before renal artery constriction in the hypertensive group(P<0.001) ; and the plasma Hs-CRP levels at the end of 8 weeks was significantly higher than that at the end of 4 weeks in the hypertensive group (P<0.001); the plasma Hs-CRP levels at the end of 12 weeks was significantly higher than that at the end of 8 weeks in the hypertensive group (P<0.001); Correlative analysis Through correlative analysis, there is clear positive correlation between SBP and vWF ( r = 0.828, P < 0. 001) ,SBP and Hs-CRP( r = 0.848, P < 0. 001) , vWF and Hs-CRP ( r =0.771, P < 0. 001).Histology in two groups No brain lesions were observed in the sham-opetated group at the end of 12 weeks after renal artery constriction. The cerebrovascular lesions of 43 rats (43/47,91.5%)were found in hypertensive group at the end of 12 weeks after renal artery constriction,mainly showed fibrinoid necrosis, hyaline degeneration, and hyperplasia of the wall of arterioles, cell proliferation in the external layer, and stenotic lumen ;and thrombotic vascular occlusions of 2 rats(2/47,4.3%)was observed in small arteries .Conclusion 1. Serum Hs-CRP level is associated with elevated blood pressure in essential hypertension.Inflammatory response might play an important role in the pathogenesis of essential hypertension. 2. When the SBP was increasing, the concenrations of serum vWF and Hs-CRP were increasing simultaneously; vWF and Hs-CRP played important roles in atherosclerosis.3. The high concenrations of serum vWF and Hs -CRP in essential hypertension were both the predictive operators to ischemic stroke.
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