The Experimental Research Of The Effect Of Mitomycin C Applied Locally In Corneal Neovascularization Of Rabbit

Objective: Research the effect of mitomycin-C on inhibiting rabbit's corneal neovascularization after alkali burning and to evaluate its toxicant side effect on cornea with control and the relationship between proliferation of corneal neovascularization and inflammatory cell inflitration and VEGF and CD34 by experimental method.Methods: 24 healthy rabbits are take into this experiments that have eyes diseases are excluded, and they are divided into two groups randomly. There are 12 rabbits in each group. The corneal neovascularization was induced on both eyes by alkali burn in 24 white rabbits. 0.9% Sodium chloride was dropped four times daily in group A, mitomycin-C was injected subconjunctival tissues in group B, pranoprofen was dropped four times daily in group C, dexamethasone was dropped four times daily in group D. The development of cornea edema dilapsus and the growth of neovascularization were observed in every other day, six rabbits in each group were put to death by injecting air in the vein of it's ear in seventh and fourteenth day, the cornea tissues were accounted by immunhistochemical analysis of Vascular Endothelial Growth Factor and CD34, hematoxylin and eosin stain under high power lens.Results: The vascular in corneal limbus become telangiectatic, and congest 24h after alkali burning. Vascular grow into cornea in 3 days, corneal neovascularization grow fast in 10 days. Cornea edema is obviously in all cases early, there are 2 cases were fond partly corneal ulcer in group A. corneal epithelium cells begin to grow several days later. We can find the corneal neovascularization were inhibited strongly in group B than it in group A,C and D, the value P were 0.000, 0.005, 0.000, 0.000 in which group B correspond to group A, the inhibit effect were stronger than dexamethasone , the value P were 0.078, 0.291, 0.193and 0.046 in which group B correspond to group D, and the immunreactivity were slower and fewer than group D. Between seventh and fourteenth day, the value P of cornea edema was 0.000,the cornea dilapsus were P7=0.000,P14=0.136,the polymorphonuclear leukocyte under hematoxylin and eosin stain were P7=0.034,P14=0.000.Conclusions: Non-toxic mitomycin-C which inject subconjunctiva has a strong effect on inhibiting corneal neovascularization after alkali burning. Proliferation of corneal neovascularization checked by biopsy and microvessel density under high power lens has a strong relationship with VEGF and CD34 in each group. Topical application of mitomycin-C has significant effects on the inhibition of corneal neovascularization, and it's toxicant side effect on cornea have no significant difference correspond to dexamethasone.