The Relationship Between Coronary Heart Disease And Polymorphism Of Angiotension Converting Enzyme And Angiotensiongen Genes

Objetive:To study the association between coronary heart disease and polymorphism of angiotension converting enzyme gene insertion/deletion(I/D)and angiotensiongen gerie M235T in Chinese people from ShanXi and vicinity.Methods:Polymerase chain reaction combined with restriction enzyme digestion(PCR-RFLP) was used to analyse the gene polymorphism in 110 patients with CHD identified by coronarty angiography and 80 control subjects that was composed of 62 cases with normal result of coronary angiography and 18 healthy subjects without clinical evidence of CHD.Results:①The frequency of DD genetype and D allele is significiently higher in CHD group than that in control group(43.6%,60.5%vs 26.3%,44.4%respectively)(P＜0.05).The frequency of TT genetype and T allele is significiently higher in CHD group than that in control group (66.4%,78.6%vs 42.5%,60.6%respectively)(P＜0.05).②In men,the frequencies of DD genetype,D allele and TT genetype,T allele are significiently higher in CHD group than that in control group(P＜0.05).In women,the frequencies of DD genetype,D allele and IT genetype, T allele have no significant difference between CHD group and control group(P＞0.05).③The odds ratio(OR)estimated by combined analysis with ACE-DD and AGT-TT genotypes(6.098) is markedly increased compared with that estimated alone from either ACE-DD(2.175)or AGT-TT(2.669)genotype.Conclusion:There is a strong correlation between coronary heart disease and polymorphism of angiotension converting enzyme gene insertion/deletion(I/D)and angiotensiongen gene M235T, and combined analysis of the ACE-DD and AGT-TT genotype may enhance the predictability of CHD.Sex may be a risk factor of CHD.