Study Of Treating Toxicosis Mucosal Lesion With Omeprazole And Prostaglandin

OBJECTIVE: The purposes of this study was to set up the modelsof the acute gastric mucosa lesion induced by methamidophos poison inrats; and to explore the treating effect of acute mucosal lesion withprostaglandin and different dose of Omeprazole (losec). Meanwhile, thechanges of Endothelin,Tumor Necrosis Factor and Cyclooxygenase-2after treatment were observed; It may provide instruction and evidenceof clinical therapy on poisoning related acute Mucosal Lesion.METHODS: Thirty six healthy SD rats were randomly divided intosix groups as below. (A)the blank control group, (B)the acutemethamidophos poison group, (C) the acute methamidophos poisongroup cured with Omeprazole of normal dose (10mg/kg), (D) the acutemethamidophos poison group cured with Omeprazole of largedose(50mg/kg), (E) the acute methamidophos poison group cured withPGE (10ug/kg), (F) the acute methamidophos poison group cured withPGE combined with Omeprazole. Each group was consisted of 6 rats.The group A were gived intragastric administration with normal salinewater 8ml/Kg-weight. The group B-F were gived with 0.2％methamidophos 16mg/kg-weight. 30 minutes after intragastricadministration, The group C was gived peritoneal injection withOmeprazole of 10mg/kg; In group D, rats were cured with Omeprazole of 50mg/kg; group E were cured with PGE of 10μg/kg; group E were curedwith PGE of 10μg/kg and Omeprazole of 10mg/kg. The rat's blood weredrowed at 4 hours after each model was established. The gastric ulcerindex was counted and the content of serum cholinesterase, blood plasmEndothelin, blood serum Tumor Necrosis Factor andCyclooxygenase-2 expression were checked.RESULTS:①Models of the acute methamidophos poison in ratswere set up successfully.②Comparison of gastric ulcer index:therapeutics group and non-therapeutics groups develop differentresults, and different therapeutics group also get different therapeuticeffects. Omeprazole of large dose and Omeprazole of normal dosecombined PGE can improve the mucosal lesion caused bymethamidophos significantly.③Pathologic changes with HE dyeing: Thegastric mucosa of groups C, D and F displayed typical improvementcompared with poisoning group B while group E had little difference.④There were similar changes on content of blood plasm Endothelin andblood serum Tumor Necrosis Factor-a between control and thetherapeutics groups. In therapeutics groups the content of blood plasmEndothelin and blood serum Tumor Necrosis Factor-a are also diversestatistically. Rats in large dose of Omeprazole get lower content of bloodplasm Endothelin and blood serum Tumor Necrosis Factor-a than that inthe normal dose group.⑤the cyclooxygenase-2 expression: the expression of cyclooxygenase-2 in the stress group were raised. The sameraise was observed in group C-F and there were no statistic differencebetween them.CONCLUSION:①The gastric ulcer index of rats in the methamidophos poisoningrats increased.②The application of Omeprazole can release acute mucosal lesioncaused by methamidophos poisoning, especially the large dose ofOmeprazole group and Omeprazole combined with PGE group.however PGE alone cannot improve the lesion obviously.③The content of blood plasm Endothelin and blood serum TumorNecrosis Factor-a increase in organophosphate poisoning related acutemucosal lesions, and they can be related to the improvements of therapy.④The COX-2 expression of rats in the stress groups raise, hint itsrelation between lesion of gastric mucosa, but the expression can not berelated to the therapeutic effect.