| Objective: To study the expression of hypoxia-inducible factor-1(HIF-1)in the braintissue and serum content of malondialdehyde (MDA) after the brain injury caused by acuteorganophosphate poisoning (AOPP), and the interventional effect and mechanism ofhyperbaric oxygen therapy, thereby providing a theoretical basis on the expansion of thehyperbaric oxygen application.Methods: Sixty healthy male SD rats were randomly divided into four groups: controlgroup (n=6), poisoning group (n=18), routine group (n=18) and hyperbaric oxygen group(n=18). The rats were injected subcutaneously with dichlorvos (4mg/kg) on the nape ofneck for a total of five times. The requirement of uninterrupted convulsions for3hours inrats must be achieved after each injection. Then the routine group was given intramuscularinjection of penehyclidine hydrochloride (0.12mg/kg) and pralidoxime chloride (30mg/kg)and the hyperbaric oxygen group received hyperbaric oxygen therapy immediately afterconventional treatments. The control group was injected of the same amount of saline. Allrats were sacrificed at1,3,7hours after model establishment (six rats at each time point)and the blood samples were taken from inferior caval vein, followed by the isolation andmeasurement of Malondialdehyde(MDA) content by using thiobarbituric acidchromatometry. After the decapitation and the removal of brains, the cortexes were fixed in4%paraformaldehyde for paraffin embedding and pathological section,then routine HEand nissl staining were performed. The expression of HIF-1protein was detected byimmunohistochemical (IHC) and the quantitative real time PCR was used to detect theexpression of HIF-1mRNA in hippocampus. All results were analyzed bystatistic software SPSS.Results: Compared with poisoning group, the pathological damage to cerebral tissueslessened in the hyperbaric oxygen group. The expression of HIF-1and MDA content inpoisoning group was much higher than that in control group (P<0.05). After the treatment of hyperbaric oxygen, the expression of HIF-1and MDA content were significantly lowerthan both that in the poisoning group (P<0.05) at all time points and that in the routinegroup at the time points of1and3hour (P<0.05). There was a positive correlation betweenthe expression level of HIF-1mRNA and the serum level of MDA by correlationanalysis (r=0.909, P=0.000).Conclusion:The efficacy of hyperbaric oxygen intervention against AOPP-induced braininjury is better than that of the routine treatment and its mechanism may be theantioxidation and the inhibition of HIF-1expression. The best efficacy will be achievedby the intervention immediately after the brain injury. |
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